中文摘要
据土家族医学"阴盛"致衰、"毒气"致病理论，提出血管性痴呆（VD）"理阴化毒"治法原则。以"理阴-调控血管生成开关-复健脑筋"、"化毒-纠正Nrf2-ARE级联GSH/GSSG失衡-祛除玍毒"为指引，评价天珠散（TZS）对自然衰老慢性低灌注型VD大鼠脑血管老化与血管新生影响，测定"血管生成开关"效应；以TZS含药血清对缺氧衰老内皮细胞（EC）ROS及GSH/GSSG系统作用反映氧化还原平衡参与机制。"权重配方法"优化TZS组成，分析其对VD大鼠脑血管新生、促/抑血管生成因子表达及TGF-β/TSP-1开关调控机制；在缺氧衰老脑EC上，观察TZS促细胞增殖、迁移及血管新生与GSH/GSSG系统平衡、Nrf2-ARE网络级联调控关系，明确TZS调控Nrf2-ARE网络纠正氧化还原失衡及促血管新生机制，进而阐明"理阴化毒"治法"理阴盛"、"祛玍毒"科学内涵，丰富土家族医学防治VD理法方药体系。
英文摘要
According to the theory of 'Yin excess' inducing senescence and 'gas' inducing diseases in Tujia medicine, the treatment principle of 'Adjusting Yin and Eliminating Toxicity' is proposed to treat vascular dementia(VD). Based on 'adjusting Yin-regulating Angiogemic Switch- restoring the brain microvascular' and'eliminating toxicity-correcting Nrf2-ARE pathway and cascade regulating imbalance of GSH/GSSG' , the influence of TianZhuSan(TZS) on the cerbral vascular aging and the angiogenesis of natural aging rat models with common carotid artery( VD rats) will be evaluated and the effects of Angiogemic Swith will be detected then. The effects of TZS medicated serum on ROS and GSH/GSSG system in the hypoxic and aging endothelial cells will indacate the mechanism of redox balance. The weight distribution method is used to optimize TZS，The cerbral angiogenesis and the expression of promoting/inhibiting angiogenesisi factors of VD rats will be influenced by new TZS, and the regulating mechanism of TGF-β/TSP-1 affected by new TZS will be analyzed. In the hypoxic and aging endothelial cells, the relationship between Nrf2-ARE network cascade regualting, the balance of GSH/GSSG system, the angiogenesis and the cell proliferation and migration influenced by TZS will be observed. The mechanism of the angiogenesis and correcting redox imbalance regulated by Nrf2-ARE network,which is influenced by TZS, will be concluded. This project will benefit to iluminate the scientific connotation of adjusting Yin excess and eliminating Gadu in the methods of 'Adjusting Yin and Eliminating Toxicity', and improve the prescription system to treat VD in Tujia medicine.