周围神经缺损的治疗是神经科学和骨科学的难题。建立“异体神经组织库”，对于周围神经缺损修复极为重要，而周围神经冷冻保存是建立“异体神经组织库”的关键。冷冻保存会导致组织细胞损伤。我们研究发现（NSFC：81373668）：周围神经冷冻保存时，川芎嗪、人参皂甙Rb1、雷公藤多甙能抑制周围神经SCs细胞凋亡，提高保存后神经生物活性，但与新鲜神经相比差距较大。本项目提出：冷冻保护剂毒性和低温缺氧，反玻璃化与稀释性渗透，可能对冷冻保的周围神经造成严重损伤，预防/减轻这些损伤，可能是周围神经冷冻保存成功关键。冷诱导RNA结合蛋白（CIRP）是一种冷应激蛋白，在低温、缺氧、渗透压应激下对机体具有保护作用，川芎嗪、人参皂甙能提高组织缺血缺氧耐受力。我们拟通过CIRP在周围神经保存时诱导表达，联合川芎嗪、人参皂甙Rb1、雷公藤多甙，来预防周围神经冷冻保存损伤，并在异体移植前对周围神经冷冻保存损伤行体外修复。

The therapy of peripheral nerve defect have always troubled neuroscience and orthopedics.It is very important for the peripheral nerve defects repair that establish the allogenic nerve tissue bank, and that the cryopreservation of peripheral nerve is the key to establish the allogenic nerve tissue bank. Cryopreservation can lead to tissue and cell damage. In our previous studies (NSFC:81373668), Ligustrazine, ginseng saponin Rb1 and tripterygium glycoside can inhibit the apoptosis of peripheral nerve SCs and increase the activity of the peripheral nerve in cryopreservation, but the defect compared with the fresh nerve is very serious. The toxic effects of CPA and the low temperature and hypoxia, as well as the anti vitrification and Dilution infiltration, may cause severe damage to the peripheral nerves in cryopreservation, and preventing the occurrence of these injuries may be the key to the cryopreservation of peripheral nerves.. Cold induced RNA-binding protein (CIRP) is a kind of cold stress protein, which has a protective effect for the body in low temperature, hypoxia and osmotic stress. Ligustrazine and panaxosides can increase tissue ischemia and hypoxia tolerance. The central theme of this project proposal is to prevent the damage of the peripheral nerve by the expression of CIRP in cold preserved peripheral nerve and the effect of ligustrazine, ginseng saponin Rb1 and tripterygium glycosides, and to repair the injury of the peripheral nerve in vitro before nerve allogeneic transplantation.