骨髓造血微环境是造血干细胞赖以增殖、分化和成熟的“土壤”，对造血细胞起着重要的支持和调节作用。本项目在前期研究的基础上，围绕骨髓造血微环境，应用流式细胞术、ELISA、荧光双标、RT-qPCR、Western-blot等多种方法检测与骨髓造血干细胞的归巢、增殖、分化、迁移等密切相关的造血细胞因子、黏附分子和微血管损伤修复的内皮细胞生长因子等指标；通过透射电镜、二维和三维形态学测量技术，观察骨髓超微结构的形态变化，从微观分子生物学和形态测量学的角度探讨针灸对环磷酰胺（CTX）化疗小鼠骨髓造血微环境修复和保护的作用机制，为揭示针灸抗放化疗毒副作用的机理提供新的实验依据。

The hematopoietic microenvironment in the bone marrow is the "soil" of the hematopoietic stem cell proliferation, differentiation and mature, playing an important role to support and regulation of the hematopoietic cells. The project on the basis of the previous research, surrounding the hematopoietic microenvironment in the bone marrow, from bone marrow hematopoietic stem cell homing, proliferation and differentiation, and migration of hematopoietic cytokines, adhesion molecules and microvascular damage of endothelial cell growth factor, etc. Application of the flow cytometry instrument, ELISA, fluorescent double labeling, RT-qPCR, Western-blot, and other methods to explore the role of the molecular mechanisms; And through the transmission electron microscopy,the 2d and 3d morphology measurement technology, observe the ultrastructural morphology changes of the bone marrow, From the Angle of molecular biology and morphometric study and discussion on acupuncture and moxibustion on CTX chemotherapy repair and protection of the mechanism of action of bone marrow hematopoietic microenvironment, To reveal the mechanism of acupuncture and moxibustion resistance to radiation and chemotherapy side effects to provide new experimental basis.