如何通过促进机体白色脂肪组织棕色化来控制肥胖是目前研究的热点，基于PGC-1α/Irisin/UCP1 信号通路在白色脂肪组织棕色化的过程中具有重要作用，为进一步阐明针灸减肥的作用机制，我们拟以肥胖大鼠为研究对象，通过电针、运动及电针结合运动干预，观测对大鼠体格、血生化相关指标等的影响，评价电针对肥胖大鼠的减肥降脂作用；通过检测电针干预后肥胖大鼠棕色脂肪含量、线粒体含量活性有氧代谢等相关指标，评价电针对白色脂肪组织棕色化的促进作用；通过采用siRNA沉默PGC-1α信号通路，进一步明确PGC-1α/Irisin/UCP1信号通路在电针促进白色脂肪棕色化的作用机制；通过观察电针配合运动对肥胖大鼠白色脂肪棕色化的协同作用，为临床采用电针配合运动治疗肥胖提供依据，从而优化临床治疗方案。

The hotspot of the research is how to control obesity through promoting browning of white adipose tissue. Based on the fact that PGC-1α/Irisin/UCP1 signaling pathway plays an important role in the process of white adipose tissue browning. To further elucidate the action mechanism of acupuncture in losing weight, we intend to take obese rats as the research object. We will apply electroacupuncture, exercise and exercise plusing electroacupuncture intervention respectively to observe their influence on rat’s weight, blood biochemical index, etc. to evaluate the effect of electroacupuncture on losing weight and lipid-reducing in obese rats. We will detect brown fat content, mitochondrial content, activity, aerobic metabolism and related indices of the obese rats after EA to find out whether electroacupuncture can promote white adipose tissue browning. We will also block PGC-1 signaling pathway by siRNA to further clarify the mechanism of PGC-1 alpha /Irisin/UCP1 signaling pathway in electroacupuncture promoting white fat browning.we will observe the synergistic effect of electroacupuncture combined with exercise on promoting browning of white adipose tissue in obese rats, so as to provide evidence for clinical use of electroacupuncture combined with exercise therapy for obesity,and clinical treatment of scheme optimization.