中文摘要
1，3-丙二醇（PDO）是合成高分子材料PTT的单体，在纺织等领域有广阔应用前景。本研究室前期获得了高产甘油的酵母；克隆并在E.col中成功表达了甘油合成PDO的关键酶基因。本项目进一步将甘油合成PDO基因导入酵母中，实现了具有以酵母为宿主、以更为廉价的葡萄糖为发酵原料的一步法合成PDO途径的构建。在此过程中，还建立了工业酵母的遗传转化方法，并对酵母甘油合成关键酶基因及其启动子功能进行了探讨。
英文摘要
1,3-propanediol (PDO) is the monomer of sythesizing new polyester poly propylene terephthalate(PTT), with broad application in fiber industry. The routine process for microbial synthesis of PDO is from glycerol by Clostridium butyricum under anaerobic condition. DuPont have constructed recombinant E.coli strain that capable of conversing glucose to PDO. However, because the strain containing too many exonous genes, it is not stabe enough to industrilize. One yeast strain overproducing glycerol was screened out previously in our laboratory. Recently, the genes encoding key enzymes for sythesis of PDO from glycerol were cloned and overexpressed in the engineered E.coli. In the current program, the genes were transferred and intergrated into the genome of yeast. The engineered yeast can produce PDO from glucose.The fermentation raw material is glucose but not more expensive glycerol. The one-step fermentation process under aerobic condition is simpler than that of two-step method under anaerobic condition, because the one-step process doesn't require anaerobic fermentation equipments and nitrogen gas supply.
结题摘要
1，3-丙二醇（PDO）是合成高分子材料PTT的单体，在纺织等领域有广阔应用前景。本研究室前期获得了高产甘油的酵母；克隆并在E.col中成功表达了甘油合成PDO的关键酶基因。本项目进一步将甘油合成PDO基因导入酵母中，实现了具有以酵母为宿主、以更为廉价的葡萄糖为发酵原料的一步法合成PDO途径的构建。在此过程中，还建立了工业酵母的遗传转化方法，并对酵母甘油合成关键酶基因及其启动子功能进行了探讨。