中文摘要
用高效好氧菌和厌氧颗粒污泥通过自固定化技术在有限供氧弱还原电位条件下组成厌氧好氧耦合生物系统,利用厌氧、好氧菌群协同作用来高效降解高氯酚,从动态角度定量化地描述厌氧好氧耦合系统中限制性生态因子(氧化还原电位ORP、pH、供氧速率、氢分压、氯酚负荷等)作用规律和调控对策,通过各种生态及环境因子,来调变各类菌群的功能与结构关系,通过微生物降解活性和代谢产物分析,并从能量代谢及电子传递效能角度揭示厌氧好氧耦合快速高效降解高氯代有机物机理,采用PCR-DGGE对同时厌氧好氧生物系统种群结构的时空分布变化原位特性进行研究,从分子生物学水平上对同时厌氧好氧降解PCP过程微生物种群结构分子生态特性及其变化状况进行研究,从群体生态学角度考察系统中优势顶极群落的形成机制及其生态演变规律,对高效降解高氯代芳香化合物菌群的驯化和富集,同时也对污染土壤和地下水的原位修复具有重要的现实意义。
英文摘要
The coupled aerobic-anaeobic system is established with co-immobilized aerobicanaerobic.microorganisms under limited aeration, the attractive efficient degradation of.highly chlorinated phenols is to be achieved with the cooperation of aerobes and.anaerobes through the aerobic-anaerobic synergism, the restrict factors ( ORP、pH 、DO and CP load) are dynamically and quantitied studied to indicate the relation of.microorganism community construction and function, the high degradation mechanism of.PCPs was also deduced from the metabolites,the specific anaerobic-aerobic activities,.energy metabolism and electron transfer. Population dynamics in the coupled systems are.followed under the same operation conditions using the polymerase chain reaction -.denaturing gradient gel electrophoresis(PCR-DGGE) method. the peak microorganism.community formation and zoology evolvement rules are also reviewed from the point of.colony ecology, which is important for the PCP dedradation microorganism.acclimatizationa, richment, in site restore of soil and underground water.
结题摘要
用高效好氧菌和厌氧颗粒污泥通过自固定化技术在有限供氧弱还原电位条件下组成厌氧好氧耦合生物系统,利用厌氧、好氧菌群协同作用来高效降解高氯酚,从动态角度定量化地描述厌氧好氧耦合系统中限制性生态因子(氧化还原电位ORP、pH、供氧速率、氢分压、氯酚负荷等)作用规律和调控对策,通过各种生态及环境因子,来调变各类菌群的功能与结构关系,通过微生物降解活性和代谢产物分析,并从能量代谢及电子传递效能角度揭示厌氧好氧耦合快速高效降解高氯代有机物机理,采用PCR-DGGE对同时厌氧好氧生物系统种群结构的时空分布变化原位特性进行研究,从分子生物学水平上对同时厌氧好氧降解PCP过程微生物种群结构分子生态特性及其变化状况进行研究,从群体生态学角度考察系统中优势顶极群落的形成机制及其生态演变规律,对高效降解高氯代芳香化合物菌群的驯化和富集,同时也对污染土壤和地下水的原位修复具有重要的现实意义。
