申请人从事植物根系发育研究十多年，揭示了姐妹染色单体的正确分离将有丝分裂和细胞极性伸长偶联；阐明了转录因子在植物胞间移动的完整机制；并发现了植物根横向结构进化的分子调控路径之一；改进了多项细胞学成像技术，并在胞间连丝调控领域取得重要进展。五年来，在PNAS等国际著名专业杂志发表论文18篇，一作文章和通讯作者（或共同）为12篇，平均影响因子为8.02。其中7篇文章被f1000推荐，两次受邀为Curr Opin Plant Biol.撰写综述。多次受邀为全美植物生物学年会(ASPB 2014)，全国植物生物学大会（2015），国际植物激素大会(2016)和冷泉港亚洲会议(2017)等做专题报告，迄今为PNAS，Plant Cell等杂志审稿31次。本项目将深入研究植物胞间连丝传递信号如何调控根系的发育，这将为揭示根系中不同类型细胞的分裂和分化模式调控的分子机理提供基础。

I have been engaged in the plant root development for over 13 years. My research identified a novel protease with an essential role in the separation of sister chromatids. In addition, I found the involvement of this protease in cytokinesis and subsequent anisotropic cell elongation. I also examined the movement of transcriptional factors between plant cells in root meristem and discovered the.endomembrane and microtubules related regulatory mechanisms. By comparative analysis of SHR and its rice homologous, I found the evolutionarily renovated intercellular dynamics accounts for the radial patterning difference between arabidopsis and rice. To help our research, I improved a number of live imaging techniques. Recently I developed a novel system in which we can efficiently.manipulate plasmodesmata. In the past five years, I have published 17 papers in mostly recognized scientific journals including PNAS. Among them, I first-authored (or as co-corresponding author) 12 papers and 7 papers got recommended by F1000. The average impact factor of my first-author papers (or co-corresponding author papers) is 8.02. I was invited twice to write reviews for Curr Opin Plant Biol.. I was also invited to give talks and seminars by internationally recognized conferences including ASPB (2014), CSPB (2015), International Auxin conference (2016) and Cold Spring Harbor Asia (2017). So far, I peer-reviewed 31 papers for journals including PNAS and Plant.Cell. In this project, I will explore how cell-to-cell communication mediated by plasmodesmata regulates root development. Specifically, I will focus on the mechanism behind cell division pattern and cell fate determination. This study will shed light on the regulatory mechanism of plant cell fate specification, as well as provide knowledge for future nutrient-efficient root system improvement in crops.