中文摘要
竹亚科香竹属是中国-喜马拉雅地区的一个特有属,以云南为分布中心,该属大多数种分布区域狭窄,具有重要的保护价值,同时还具有经济价值和生态价值。前期研究表明香竹属为单系,具有复杂的进化历史,种间遗传差异和形态差异均较小,种间界限模糊。一方面与该属为近期分化有关,另一方面可能与物种划分是否合理有关。为了进一步检验香竹属物种划分的合理性,本项目拟按照居群方法采样,观察相关形态特征,分析种间和种内形态变异;利用基因组浅层测序方法获取叶绿体基因组和核核糖体序列,同时利用二代测序技术获得50-60个低拷贝核基因片段,推测物种划分的合理性;基于野外采集和标本信息,利用软件检验种间生态位是否有分化,预测潜在分布区,为该属的保护和利用提供一定依据。通过上述研究,期望澄清香竹属的物种划分问题、分类学问题和种间关系,为竹亚科其他类群的物种划分研究提供新的思路和方法,为生物多样性保护提供更可靠的基础数据。
英文摘要
Chimonocalamus (Bambusoideae) is a genus endemic to the Sino-Himalayan region, with Yunnan as the distribution center. Most species of this genus are distributed narrowly and should be protected. Those species are also economically and ecologically important. The previous studies indicated that Chimonocalamus was monophyletic with a complex evolutionary history, while genetic divergence as well as morphological differentiation was little, and species boundaries were ambiguous. The recent diversification of this genus may be responsible for the ambiguous species boundaries, however, the unreasonable species delimitation is probably another reason. In this project, the species delimitation of Chimonocalamus will be examined by integrative methods. Based on observation and measurement of key diagnostic characters, the morphological differentiation will be assessed between inter- and intra- species. The whole plastid genomes and nuclear ribosomal DNA regions will be retrieved through genome skimming method, and about 50-60 low copy nuclear genes will be amplified by PCR and be sequenced by Illumina MiSeq. The species delimitation and phylogeny of the genus Chimonocalamus will be analyzed and reconstructed. The niche divergence between various species will be examined and the potential distribution area will also be predicted using Maxent, based on field collection information, herbarium records, and downloaded climate data. Through the comprehensive studies from morphology, phylogenomics, and ecological niche modelling, we expect to make a relatively reasonable and practicle species delimitation system for Chimonocalamus and that these methods can be applied to other bamboos.
