癌症严重危害人类健康，其发病率呈逐年上升趋势。早诊断和早治疗对癌症预后有重要的意义，肿瘤标志物的检测在癌症早期诊断中意义重大。常规肿瘤标志物检测方法存在成本高、检测时间长等缺点，电化学生物传感器检测方法可弥补常规检测的不足。本研究基于性能优异的多功能金属有机框架（MOFs）复合材料构建电化学免疫传感器用于肿瘤标志物的检测。研究内容包括：制备多功能MOFs，并与性能优异的碳纳米材料、量子点和纳米金属氧化物等合成复合材料；通过各种表征技术分析不同的合成方案和合成条件对复合材料吸附、导电及催化性能的影响；选择性能优异的复合材料构建传感器，全面系统的对传感器进行评价与优化，并对CEA，Cyfra21-1，AFP，PIVKA-II等肿瘤标志物进行检测。该项目是集电化学、免疫学和材料科学等多学科交叉的前沿性研究，可建立一种集识别、检测一体化的快速检测技术，并可为人群大样本肿瘤标志物的筛检提供技术支持。

Cancer severely threat to human health, the incidence of cancer is upgrading in recent years. Early diagnosis and early treatment have important significance for cancer prognosis. Generally traditional methods to detect tumor markers are limited due to their high cost and time consuming, while electrochemical biosensor can make up for the inadequacy of conventional detection. This study is based on the performance of the multifunctional metal organic frameworks (MOFs) composite construction of electrochemical immunosensor for the detection of tumor markers. The study includes: Excellent property carbon nanomaterials, quantum dots and nano metal oxide are combined with multifunctional MOFs to compose composite materials. Characterization technology are employed to analysis the influence of synthesis programs and conditions on the property of composite adsorption, electrical conductivity and catalytic performance. The excellent performance of composite was selected to fabricate the electrochemical immunosensor. The sensor was evaluated and optimized systematically to detect CEA, PIVKA-II, AFP, Cyfra21-1. The study is an interdisciplinary forefront research related with electrochemical analysis, immunology and materials science. We aimed to establish an integration fast detection techniques and to provide technical support for the large sample screening of tumor markers.