蚊媒病毒感染后，患者的病毒血症期短、病毒载量低以及免疫交叉反应等因素给蚊媒传染病的检测带来极大的挑战。本研究采用共价偶联技术将石墨烯组装于SPRi芯片表面，并利用PEG高分子对石墨烯表面进行化学修饰，解决传统SPRi因灵敏度的局限性以及非特异性吸附问题而无法应用于复杂样品中痕量检测的困境; 其次，利用抗原表位筛查技术对蚊媒传染病病原体所编码蛋白的抗原表位进行预测和筛选，确定可以区别蚊媒传染病的表位多肽组合，并选择具有临床诊断意义的多肽组合，制备成高内涵的多肽芯片，解决蚊媒传染病免疫交叉反应问题。通过高内涵多肽抗原芯片与高灵敏度的SPRi检测平台的强强联合，从而实现对蚊媒传染病的快速，准确的分析，为蚊媒传染病的防治工作提供新的研究工具。

Laboratory mosquito-borne diseases infection diagnosis is challenging because of low viremia, low virus RNA load in serum samples and cross-reactivity of antibodies between flaviviruses. This research develops a new method for the covalent attachment of GOSs to SPR chip, which leads to a significant increase of the SPR signal. Using PEG polymer to modify the GOSs surface to reducing non-specific adsorption. Therefore, the design of surface chemistry allows to detect trace amounts of biomarkers in complex biological media. We also apply an antigen epitope screening platform to analyses the antigenic epitopes of the coding proteins of the mosquito-borne diseases. Through three steps: antigen peptide library construction, high throughput seroscreening, and diagnostic epitope combination validation, to obtain some potential peptide probes for diagnosis of mosquito-borne diseases. The graphene-based SPRi provide a label free platform for the detection of antibodies with peptide chip. The assay is rapid, sensitive, and specific to detect mosquito-borne diseases infection. This research has the potential to provide new tool for mosquito-borne diseases prevention and control.