药用植物活性物质的发掘和利用是当前研究的重点。植物腺毛被誉为“化学工厂”，是合成和贮存各种类型和功能天然产物的重要场所，是植物抵御病虫害进攻的“第一道防线”，其中蕴含了丰富的天然活性物质。唇形科药用植物在我国医药领域具有重要地位，富含腺毛，但其中的天然产物及生物活性大多未知。前期首次建立了激光显微切割-超低温核磁共振-超高压液相色谱/质谱联用新技术方法，成功实现了对多种植物腺毛中的化学防御物质的精确研究。本项目拟选取6-8种重要唇形科药用植物，系统开展其腺毛中的天然产物分析、分离、鉴定和富集，对主要天然产物进行抗微生物和抗虫生物功能测定以及广泛的药理活性筛选，揭示腺毛中的化学防御物质，发现有应用前景的天然活性物质，为创新天然药物或农药研究提供目标活性分子，并为这些重要的药用植物资源的合理持续利用提供科学依据。

Discovery and utilization of bioactive substances of medicinal plants are a major research topic nowadays. Plant glandular trichomes, well known as “chemical factories” and “the first defense line” of plants against insect herbivores and pathogens, are the major sites for synthesis and accumulation of various types of functional natural products, which are also a rich source of bioactive compounds. Medicinal Labiatae plants have played an important role in domestic medical and pharmaceutical fields. These plants are rich in glandular trichomes but their accumulated natural products and biological functions are largely unknown. In our previous work, we pioneered the establishment of a brand new technology, laser microdissection in combination with cryogenic nuclear magnetic resonance and ultra performance liquid chromatography coupled with mass spectrometry (LMD-cryoNMR-UPLC/MS/MS) and the corresponding method, and realized the precise investigation on the defense chemicals accumulated in specific glandular trichomes of a number of plant species. Based on this, the current project aims to systematically investigate the natural products in the glandular trichomes of 6 to 8 selected Labiatae plants with important medicinal value. Qualitative and quantitative analyses, isolation, identification, and enrichment of these natural products will be carried out. Major trichome natural products will be subjected to anti-microbial, anti-insect, and extensive related pharmacological assay subsequently. The results of this project are expected to reveal the defense chemicals in the glandular trichomes of these important medicinal plants. Discovery of bioactive natural products with application potential is also anticipated, thus providing target molecules for deep new drug or pesticide research and development, and also scientific basis for the rational and sustainable utilization of these important medicinal plant resources.