铝毒是影响南方红黄壤地区花生产量的重要原因，对铝诱导PCD信号机制的研究有助于阐明铝胁迫机理。类受体蛋白激酶（RLK）在植物生长发育和响应环境胁迫的信号传导途径中发挥重要作用，本项目在对前期铝胁迫花生根尖转录组数据分析的基础上，关注RLK在铝诱导PCD中的作用，拟开展以下研究：①利用RACE技术获取响应铝胁迫的花生RLK基因全长序列，并用体外磷酸化技术验证其生化功能；②运用过表达技术，结合组织化学染色、qRT-PCR以及western blotting技术研究相关RLK在铝诱导PCD中的调控功能；③利用不同信号通路抑制剂开展抑制剂实验，并分别通过原生质体瞬时转化技术与酵母双杂交文库筛选技术分析重要RLK的亚细胞定位与互作组分，分析它在铝诱导PCD信号通路中的作用。以揭示类受体蛋白激酶在花生铝诱导PCD中的调控机制，为阐明铝诱导PCD的信号机制、改良花生品种提供理论依据。

Aluminum (Al) toxicity is an important factor that affect the yield of peanut (Arachis hypogaea) in red-yellow soil of south China. Revealing the signal transduction process of Al-induced programmed cell death (PCD) in peanut is helpful to finally elucidate the mechanism of Al stress. Receptor-like protein kinases (RLKs), as important factors in signal transduction, play critical roles in plant growth and development as well as in the response to many environment stresses. Based on the analysis of peanut root tip transcriptome data which acquired in our previous study, this project is going to study the role of RLK in Al-induced PCD process in peanut and carry out the following researches. 1, Clone the full-length cDNA sequence of Al-stress Associated RLK genes by RACE, and verify the RLKs biochemical function by in vitro phosphorylation experiment. 2, Combining the results of different experiments, including histochemical staining, qRT-PCR and western blotting, systematically analyze the effects of overexpression of RLKs on Al-induced PCD in transgenic plants to investigate the roles of RLK in regulation of Al-induced PCD. 3, Perform inhibitor experiments with different signal pathway inhibitors, and perform a yeast two-hybrid screen as well as protoplast transient transformation to reveal the interaction partners and subcellular localization of important RLK to study the roles of RLK in Al-induced PCD signal pathway. This work will reveal the regulation mechanism of Receptor-like protein Kinases in Al-induced PCD in Arachis hypogaea, and provide the theoretical basis for clarifying the signal mechanism of Al-induced PCD and improving the varieties of peanut.