COG（conserved oligomeric Golgi）是拴系COPI(coat protein complex I)囊泡的复合体，在高尔基体的逆向运输发挥重要作用。我们前期研究表明植物COG亚基COG3和COG8可以调控高尔基体的运输和形态结构，并影响花粉管的生长，但其分子调控机制还不清楚。该项目计划通过多种研究方法（细胞，遗传，分子与生化）对由酵母双杂交筛选得到1) COG5与Rab、β-COP；2）COG3与SYP31、SYP32的互作及其相关突变体展开研究，解析基于COG复合体的高尔基体形态维持和囊泡运输的分子网络，提高人们对植物高尔基体这一细胞器的认识。

COG complex is a tethering factor for COPI vesicles, and plays an important role in Golgi retrograde transport. We have recently characterized COG subunits COG3 and COG8. cog3-/+ and cog8-/+ mutants are both male sterile, mutant pollen displayed altered Golgi morphology, defective in tethering of COPI, and deficient transport of cell wall materials to the pollen tube tip, but the molecular mechanism remain elusive. Therefore, the central goal of this proposal is to illustrate the molecular network related to the plant COG complex using a combination of molecular and biochemistry, cell biology and genetics approaches. Specific objectives are to characterize the interactions between COG5 and Rab、β-COP；COG3 and SYP31、SYP32 , and their significance in Golgi morphology maintenance and vesicle trafficking. In doing so, we hope to improve our understanding of plant Golgi apparatus.