有些植物的花器官能够主动产生大量热能，这种类似于动物的生热行为具有多样的生物学功能，对生热植物的生殖发育至关重要。然而，有关开花生热的调控机制迄今尚不明确，尤其是针对线粒体功能动态决定的开花生热能量供应体系，缺乏系统的深入研究。针对前人工作的不足，本项目将以玉兰为材料，运用高分辨红外微距热成像技术、超高时空分辨率双光子显微成像以及结构光学显微成像(SIM)等新技术，着重开展以下研究：（1）关键生热部位的精确定位和活体生热细胞的精准获取（2）生热细胞内线粒体形态学变化与动力学行为分析；（3）以线粒体炫为表征的线粒体生理动态在开花生热中的功能分析；（4）与线粒体炫紧密相关的AOX和UCP等关键基因的表达模式分析。综合上述研究，系统分析线粒体功能动态对于开花生热的调控作用，为深入揭示植物开花生热调控网络奠定基础。

Flowers can produce a considerable amount of heat in some plants; this animal-like behavior plays critical roles in reproductive development of thermogenic plants via multiple biological functions. However, regulatory mechanisms underlying floral thermogenesis remain elusive. Especially, there is a growing need to systematically study the energy supply system dependent on functional dynamics of mitochondria. In consideration of the insufficiency of previous investigations, we will use Magnolia denudata (Magnoliaceae) as the material to (1) precisely identify and sample key thermogenic tissues and cells; (2) analyze morphological and distributional dynamics of mitochondria in thermogenic cells; (3) explore the functions of mitochondrial dynamics represented by mitoflash during floral thermogenesis; (4) analyze expression patterns of mitoflash-related genes, including AOX and UCP. In this project, we will apply several new technologies, including high-resolution infrared thermography, ultrahigh spatial-temporal resolution two-photon microscopy, and structured illumination microscopy (SIM). The results of this project will lead to a systemic view into regulatory roles of functional dynamics of mitochondria in floral thermogenesis, which is fundamental for unraveling the whole regulatory framework of floral thermogenesis.