土壤盐渍化严重影响棉花产量和品质，揭示棉花耐盐机理为棉花耐盐性种质改良，有效利用盐碱地提供理论基础。申请人构建了基于病毒诱导基因沉默体系(VIGS)的棉花cDNA 文库，并筛选获得敏盐基因GhPTP1。VIGS-GhPTP1棉花植株对盐胁迫耐受性增强。利用棉花酵母双杂cDNA文库，获得与GhPTP1互作的膜联蛋白GhANN5。本项目拟分析棉花GhPTP1和GhANN5基因沉默植株以及转基因拟南芥植株的耐盐特性，明确GhPTP1和 GhANN5在耐盐信号途径中的作用机制；鉴定盐胁迫下激活GhANN5的蛋白激酶，阐明GhPTP1与GhANN5互作模式，揭示由蛋白激酶和磷酸酶GhPTP1动态调控GhANN5磷酸化水平的耐盐新机制；利用磷酸化蛋白质组学以及转录组学手段，挖掘GhPTP1介导的棉花耐盐信号调控网络。研究结果将会揭示GhPTP1调控棉花耐盐新机制，为棉花耐盐分子育种提供优良的候选基因。

Cotton (Gossypium spp.) production is limited by various abiotic stresses, in particular salinity stress, which cause substantial losses of cotton yield and fiber quality worldwide. Thus, to understand how cotton responds to salt stress at the genome and proteome level and leverage genetic resources for developing tolerant cotton cultivars is essential to sustainable cotton industry and reducing the tension between crop production and available soil supply. With the previous screen of cotton virus-induced gene silencing (VIGS) cDNA library, GhPTP1 was picked out for silencing of GhPTP1 strongly enhanced salt tolerance of cotton seedlings. Both shoot and root biomasses were dramatically increased compared with the biomasses of Ctrl seedlings. GhPTP1 gene expression was also induced under salt treatment. Moreover, by using yeast 2 hybrid (Y-2-H), GhANN5 was pulled out interacting with GhPTP1. For the further study, our objectives will be a) By silencing of GhPTP1 or GhANN5 with VIGS, analysis the physiological and biochemical indexes of salt tolerance to reveal the gene function of GhPTP1 and GhANN5 in response to salt stress. b) Identify the upstream kinase phosphorylating GhANN5 upon salt stress, and demonstrate the molecular mechanism of the interaction between GhANN5 and GhPTP1, and uncover the dynamic phosphorylation process of GhANN5 modulating by kinase and GhPTP1. c) By phosphoproteomics and RNA-seq, dissect GhPTP1-mediated regulatory signaling network in response to salinity. Our proposed work will not only provide novel insights into understanding the molecular and biochemical processes under salt stress in cotton, but also supply the genetic resources for developing drought tolerant cotton cultivars.