氨基酸的分析在医药、食品、疾病诊断等领域具有重要意义。随着氨基酸的广泛应用，对氨基酸分析方法的研究与改进得到人们高度重视。毛细管电色谱（CEC）是一种高效、快速、微量、低成本的电分离分析技术，在复杂基质氨基酸的分析中具有优势。CEC研究的核心内容之一是制备具有高选择性、高分离效率的毛细管柱。项目在前期工作的基础上，提出含官能团共价有机框架（COFs）微柱制备思路：开展基于COFs的新型开管毛细管电色谱柱技术及其应用研究；拟采用生物仿生聚多巴胺功能化修饰结合层层自组装法，或3-氨基丙基三乙氧基硅烷“一步法”，固定含碳链有机硼酸配体COFs，将其用作新型毛细管电色谱固定相；该COFs固定相结构中既有亲水的硼酸基团，一定条件下可与糖蛋白等含邻二羟基的分子特异性结合，又有疏水的苯环和碳链，适用于氨基酸、多肽、蛋白质等多种非极性或弱极性化合物分离。项目的完成能够为复杂体系微量氨基酸分析提供技术支持。

The analysis of amino acid is significant in the field of medicine, food and disease diagnosis. Scientists pay more attention on the development and improvement of the analytical methods to analysis amino acids with it is widely used in the related areas. Capillary electrochromatography (CEC) is an electro-driven separation technique with virtues such as high separation efficiency, fast, microscale, low-cost, which has advantages for amino acids analysis in complex matrix samples. The fabrication of capillary column with high separation selectivity and high separation efficiency is one core research field in CEC. In this project, we will raise some thoughts for preparing micro-column modified with covalent organic frameworks base on our previous research: To carry out study on novel open tubular CEC column technology based on functionalized COFs and its application; we propose to immobilize boron COFs containing carbon chains serve as electrochromatographic stationary phase by bio-inspired polydopamine-supported technology combine with layer-by-layer method, or 3-Aminopropyltriethoxysilane assisted one-step process; both hydrophilic boric acids and hydrophobic benzene ring and carbon chains are exist in the frameworks of the COFs, which not only stick to specific glycoprotein with adjacent hydroxyl groups under the certain conditions, but also suitable for separation of numerous nonpolar and weak polar compounds, such as amino acids, peptides, proteins etc. The results of the project will provide a new methodological for microscale analysis of amino acid.