麻醉药物浓度个体化监测是临床长期以来的需求，目前还没有快速、简便、灵敏的血药浓度即时现场监测手段。本项目以目前国内外应用最广泛的现代静脉麻醉药异丙酚作为研究对象，在课题组前期研究的基础上，建立即时监测体内麻醉药的技术和方法。利用分子印迹聚合物特异性识别和捕获目标分子的能力，制备特异性分离前处理生物样品中异丙酚的分子印迹传感器；合成异丙酚石墨烯量子点纳米荧光探针，建立高灵敏度的异丙酚石墨烯量子点荧光猝灭快速检测方法。将上述即时前处理和检测方法集成于微流控芯片，实现复杂生物样品中异丙酚在线快速分离、检测，对药物体内行为进行及时评价，达到用药合理、安全、有效的目的。同时建立平行对照方法，对实验结果在临床应用前景进行评价。本课题涉及多个学科，研究成果进一步扩展，可应用于药物的临床过程监测。

Individual monitoring on anesthetic concentration has always been a long term requirement for clinical point-of-care testing (POCT). By far there is no effective, simple, sensitive POCT method to monitor drugs and blood concentrations. The project chose propofol, which has been widely used as an intravenous anaesthetic, as a research target to establish a POCT technique on drugs monitoring based on the preliminary results from the research group. First, prepared molecular imprinted biosensors with high selectivity in propofol within the pretreatment biological samples by using molecular imprinted polymer (MIP)’s specific ability of recognizing and capturing target molecule. Second, established a rapid detection method of fluorescence quenching with a high sensitivity of graphene quantum dots on propofol by synthesizing graphene quantum dots nano-fluorescent probes. After that, this study combines the pretreatment and detection methods into a microfluidic chip in order to realize rapid on line separation and detection of propofol within complex biological samples. This will immediately evaluate drug actions in vivo to reach the goal of rational, safe and effective dosage. Meanwhile, a parallel control method will be established to evaluate the clinical application of the experimental results. This project involves multiple disciplines, and can be used in the clinical process monitoring if further extends the research results.