爬山虎系葡萄科爬山虎属具园林绿化、生态修复及药用的植物。有关无机离子、内源激素及相关基因表达与调控对多年生未破茎未离体爬山虎生长茎内不定根原基诱发的机理研究，能有效提升其在园林绿化、生态修复、药用等方面的应用。本项目在前期研究发现反分化前薄壁细胞区域锌、钙、色氨酸、蛋白质、生长素、细胞分裂素等的浓度先后增高的基础上，拟用组织化学定位与检测分析，结合实时荧光定量PCR、植物显微、荧光显微与超微等技术，研究诱发爬山虎多年生未破茎未离体生长茎内薄壁细胞反分化成次生分生细胞，进而形成不定根原基，最终产生不定根的诱发因子，并探明各诱发因子间的互作关系。该研究不仅有助于揭示无机离子、内源激素及其基因表达的机制，并能丰富木本植物茎生根的基础理论，而且还能为爬山虎多年生茎自然生长时均产生不定根的机理研究和全茎生根爬山虎新品种选育、驯化、繁殖提供重要的参考。

Parthenocissus Tricuspidata Planch (Vitaceae), which is an important medicinal plant species, has a wide application in landscaping and ecological remediation. The study on mechanism of inducing adventitious root primordium in vivo within stem of perennial P. Tricuspidata by inorganic ion, endogenous hormones, and related gene expression and regulation can effectively improve the application of this plant in landscaping, ecological remediation and drug use. In the preliminary study, project group found that the concentrations of zinc oin, calcium oin, tryptophan, protein, auxin and cytokinin rised in the region of parenchymal cell before dedifferentiation. Based on this findings, project group intend to study the process of inducing retrodifferentiation of parenchymatous cell within unbroken and growing stem of perennial P. Tricuspidata to form secondary meristems, and then form the adventitious root primordium, which finally produce the inducer of adventitious root, and further to ascertain the relationships among the inducers, through applying the histochemical localization, detection and analysis, real-time fluorescent quantitative PCR, plant microscopy and ultramicrotechnique. This study not only can aid to unravel the mechanism of inorganic ion, endogenous hormones and related gene expression, and enrich the basic theory about root generation from stem of woody plant, but also can provide important reference for mechanism study of the adventitious root generation of perennial stem in P. Tricuspidata natural growth process, as well as for the introduction domestication and reproduction of new varieties of whole stem rooting.