竹红菌素是从竹黄菌（Shiraia bambusicola）子座(子实体)中分离发现的新型光敏药物，真菌子实体中常具有丰富的伴生细菌，探讨伴生细菌对竹黄菌竹红菌素合成的调节作用，对挖掘细菌-真菌间相互关系具有重要的理论意义和应用价值。我们以课题组筛选和选育的竹黄菌及其伴生细菌为对象，分析伴生细菌的多样性和伴生特性，通过共培养体系考察伴生菌对竹红菌素合成的调节作用。通过基因差异表达分析，获得竹黄菌中对伴生菌诱导有响应的竹红菌素合成特异酶基因，完善竹红菌素合成途径。通过转录组挖掘，发现伴生菌激活的竹黄沉默基因簇和诱导产生的新型蒽醌类光敏剂；构建竹红菌素发酵生产的混合培养技术，为竹红菌素等真菌光敏药物的生物技术生产提供理论基础和参考。

Hypocrellins, naturally occurring perylene quinones isolated from fungal stroma (fruiting-body) of parasitic Shiraia bambusicola, are new type of non-porphyrin photodynamic therapy (PDT) agents. There are rich bacteria associated with fungal fruiting-body in many improtant medicnal fungi including S. bambusicola. It is of scientific significances to explore the role of associated bacteria on hypocrellin biosynthesis of S. bambusicola. The research on fungal-bacterial interaction (FBI) may result in unique contributions to a novel strategy for the production of PDT drugs. We will focus our research efforts on the higher hypocrellin-yielding strain of S. bambusicola and the bacteria associated with fungal fruiting body. The structure, distribution and diversity of the bacterial communities in the fruiting body will be investigated. In the co-culture system including in vitro fungal–bacterial confrontation bioassay and co-inoculation in submerged cultures, the strains of the associated bacteria to promote hypocrellin biosynthesis will be screened. The analysis on differential gene expression and the characterization of some enzymes responding to the living bacteria will shed the light on the pathway of hypocrellin biosynthesis. With the transcriptome mining technique, we wish to find the clues for activating silent fungal secondary metabolite gene clusters by associated bacteria and producing novel bioactive perylenequinones in S. bambusicola cultures . The methods for eliciting anamorphosis fermentation will be established for higher hypocrellin yields. We believe our ongoing results will be helpful in the application of associated bacteria on biotechnological production of hypocrellins and more new photosensitivity perylenequinones. Our research will give hints or theoretical basis of the modulation of associated bacteria on mycelium cultures and cultivation of important medicial mushirooms for the source of PDT drugs.