我国小麦籽粒Zn含量及人体利用率较低对庞大人群健康构成严重威胁。与单独喷Zn相比，把喷Zn与农药及无机磷进行配合实施已成为小麦籽粒锌生物强化的最新尝试，故揭示该过程科学机制具有重要意义。本项目从“形成Zn-农药复合物与否可能会影响Zn-农药配合喷施的锌强化效果，减少Zn2+与磷酸根直接接触可能会克服Zn-P-农药三者配合的P-Zn拮抗，配合喷施可通过降低植酸含量提高锌利用率和品质”三条科学假设出发，拟采用田间及培养试验方法，并应用68Zn稳定性同位素示踪、LA-ICP-MS等技术，一是进行Zn与农药配合喷施对Zn在小麦体内吸收运转及分配的影响研究，揭示该过程影响籽粒锌强化效果的机制；二是阐明Zn-农药-P配合喷施中克服喷施各环节中P-Zn拮抗作用的机制；三是查明配合喷施对于小麦籽粒人体利用率及品质的影响。研究结果为喷Zn与病虫害防治一体化下实现小麦籽粒锌强化目标提供科学依据。

Low Zn concentration of wheat grain and its low utilization effeciency in human being is a major public health problem in China. Currently, spraying Zn combined with pesticides and inorganic phosphorus has become a new global challenge and topic for achieving Zn biofortification of wheat grain as well as its mechanisms compared with spraying Zn alone. Here, we hypothesized that the Zn-pesticide compounds derived from spraying Zn combined with pesticides influence on Zn biofortification of wheat grain, and P-Zn antagonism was alleviated by the addition of pesticide in the spraying solution, resulting in reducing the concentration of phytic acid in wheat grain and consequently hancing the Zn utilization effeciency in human. Based on the mentioned hypothesis, the field and incubation experiments will be conducted and some new technologies such as 68Zn isotopic tracer and LA-ICP-MS will be also used as research methods. Accordingly, this project is objective to reveal the mechanism for Zn biofortification of winter wheat through investigating the effect of spraying Zn combined with pesticides on Zn uptake, transportation, and distribution, understand the reason for alleviating the P-Zn antagonism by spraying Zn combined with pesticides and inorganic phosphorus, and clearify the effect of the combined spraying practice on Zn utilization effeciency in human being. Eventually, these results will provide the guidance for achieving Zn biofortification of wheat grain as well as pest control.