迄今对植物耐盐生理及分子机制的研究大都集中在Na+毒害和适应方面,但植物盐害的阴离子毒害主要由Cl-造成,一直为多数研究学者所忽视。栽培大豆的Cl-胁迫大于Na+,而盐生野大豆对Cl-表现出颇强的抗性。与大豆植株体内Cl-吸收、转运及分子调控密切相关的氯离子通道蛋白(CLCs)做为阴离子通道或转运蛋白,如GmCLC1,已表明参与了盐逆境下植株体内Cl-稳态和耐氯(盐)性调控。本项目重点关注栽培和野生大豆氯(盐)害的生理响应差异和CLCs同源基因的发掘,通过基因克隆、细胞器标记基因编码蛋白亚细胞共定位、酵母(突变体)互补、大豆转基因(发根农杆菌侵染、根癌农杆菌子叶节诱导)和病毒诱导的基因沉默（VIGS）技术等多种分子生物学手段,解析和揭示引起栽培和野生大豆耐氯(盐)性差异的关键CLCs同源基因及其生理功能,以期为今后用于改良大豆或其它作物的耐氯(盐)性提供重要的科学依据。

So far, the study on physiological and molecular mechanisms of plant salt tolerance mostly focus on Na+ toxicity and adaptation, but anionic toxicity of plant salt injury is mainly caused by Cl-, and Cl- toxicity has been yet ignored by most researchers. The effect of Cl- stress on the cultivated soybean is greater than that of Na+ stress, while wild soybean shows a better resistance to Cl- stress. As a kind of anion channels or transporters, the chloride channel proteins (CLCs), which are closely related to the absorption, transportation and molecular regulation of Cl- in soybean plants, such as GmCLC1, are involved in regulation of Cl- homeostasis and chloride (salt) tolerance under salt stress. This project will focus on the physiological response difference of chloride (salt) injury on cultivated and wild soybeans, and whole soybean genome discovery of CLC homologous genes. By adopting multiple analysis tools of molecular biology, such as CLCs gene cloning, subcellular co-localization of CLCs protein with proteins encoded by organelles marker genes, yeast mutant complementary test, soybean plant genetic transformation (including transgenic hairy roots mediated by Agrobacterium rhizogenes, cotyledonary node tissue transformation mediated by Agrobacterium tumefaciens) and virus induced gene silence (VIGS), the key CLCs homologous genes and their special physiological functions related to the chloride (salt) toxicity difference between cultivated and wild soybeans will be revealed, and we hope to provide the important scientific theory basis for future utilization of the key CLCs homologous genes on chloride (salt) resistance improvement in soybean and other crops.