我们前期工作表明，ZmCCaMK在BR诱导的抗氧化防护及增强作物耐旱性中起重要作用，进一步筛选到可能与ZmCCaMK存在直接相互作用的蛋白质---ZmBSK1。本项目拟在前期工作的基础上，进一步验证ZmCCaMK与ZmBSK1之间的相互作用，鉴定ZmCCaMK与ZmBSK1底物磷酸化位点；利用ZmCCaMK、ZmBSK1过表达与基因沉默、底物磷酸化位点突变的转基因材料结合瞬时体系，明确ZmCCaMK与ZmBSK1相互作用在BR诱导的抗氧化防护中的作用及其信号转导机制、与BR诱导的H2O2的关系及在玉米耐旱性中的作用。最终阐明ZmCCaMK与ZmBSK1相互作用在BR诱导抗氧化防护进而增强作物耐旱性中的作用，进一步明确CCaMK参与植物抗逆反应的作用机制。这一研究的开展不仅有利于人们理解逆境下BR诱导抗氧化防护信号转导机理，而且也对于人们利用分子生物学手段提高作物的耐逆性具有重要的指导意义。

Our previous study showed that ZmCCaMK plays important roles in BR-induced antioxidant defense and crop drought tolerance. Further, an interacting protein of ZmCCaMK---ZmBSK1 was screened. In this project, based on our previous studies for this project, we intend to identify the interaction between ZmCCaMK and ZmBSK1, and to clarify the phosphorylation sites of substrate. Further, we want to elucidate the role of ZmCCaMK interacting with ZmBSK1 in BR-induced antioxidant defense, BR-induced H2O2 production and drought tolerance by using ZmCCaMK and ZmBSK1 overexpressing or silencing, direct mutation of substrate phosphorylation sites overexpressing transgenic maize, with transient overexpressing or silencing test in maize protoplasts. Finally, we shall clarify the roles of ZmCCaMK interacting with ZmBSK1 in BR-induced antioxidant defense and drought tolerance, and further clear the mechanisms of CCaMK in plants under stresses. This project is not only helpful to our understanding the mechanisms of BR-induced antioxidant defense signal transduction, but also has an important significance for improvement of crop stress tolerance by using molecular biology manipulation.