动眼神经麻痹（Oculomotor Nerve Palsy, ONP）是常见的脑神经疑难疾病，多继发于脑部病变和代谢性疾病，临床表现为复视、眼球运动障碍等，给患者造成巨大的身心痛苦。目前ONP功能修复已引起国际医学界的高度关注，然而，有效的西医康复手段尚未见报道。本课题组自2007年率先采用眶内电针方法治疗ONP患者1032例，初步分析显示：该方法可大幅改善甚至消除患者ONP症状，有效提高其生存质量。为从机理上阐明该方法的有效性，本项目拟采用雄性SD大鼠为实验动物，以神经再生过程中发挥重要作用的巨噬细胞M2型极化为研究目标，采用免疫荧光标记、蛋白印迹等方法进行检测，通过记录神经生长因子、免疫反应因子的蛋白及基因表达水平，从分子层面阐释眶内电针在ONP功能重建过程中的作用机制，为实现该方法在临床上的推广应用提供科学依据，同时，为提升针灸治疗疑难杂症在医学界中地位奠定必要的技术储备。

Oculomotor nerve palsy(ONP), as a commonly seen but complicated cranial nerve neuropathy, is usually secondary to cerebral and metabolic disorders. The patient would be tortured physically and psychologically due to the manifestations include diplopia and ocular motility disorders. Nowadays, functional reconstruction of ONP has been highly-focused by researchers from international medical community. However, efficient rehabilitation method hasn’t been reported yet. Our team first adopt the intraocular electroacupuncture therapy into ONP rehabilitation and has treated 1032 patients since 2007. By statistical analysis, the results indicate that this therapy could remarkably relieving or even eliminating the symptoms and effectively improving the patient’s quality of life. In order to elucidate the actual mechanism of the therapy, we would use SD rat as the object to observe the macrophages M2 polarizing which play a notable role in nerve regeneration during intervening by intraocular electroacupuncture. We would apply western blot and RT-PCR technique to investigate the protein and mRNA expression level of immune reactive and neurotrophy factors, by which to elucidate the molecule mechanism of intraocular electroacupuncture interfereing the functional reconstruction of ONP. This project could not only provide scientific evidence for promoting the therapy into clinical practice, but also lay the technical foundation for upgrading the position of acupuncture in treating incurable diseases in medical world.