植物病原微生物利用效应因子操纵植物细胞并建立定殖，因此根据效应因子转运机制开发阻断技术对于病害防治具有重要价值；然而目前真菌效应因子转运机制研究滞后，既没有发现保守的寄主定位信号，也不清楚效应因子如何跨越寄主细胞膜。.近年来以卵菌RxLR效应因子寄主定位信号为切入点的研究，揭示了磷脂酰肌醇3磷酸介导的跨膜转运机制。申请人在杨树病原真菌-杨盘二孢菌中定义了IGY蛋白家族，其与卵菌RxLR效应因子家族具有广泛相似性。本研究将进一步分析IGY蛋白跨膜转运机制，主要内容是：蛋白组学技术鉴定进入杨树细胞的IGY蛋白，蛋白杂交技术验证寄主定位信号，活细胞成像技术分析IGY蛋白在侵染过程中的动态变化，脂质组与生物大分子相互作用技术判断IGY蛋白跨越杨树细胞膜的具体途径，进而通过筛选抑制剂和杨树转基因阻断IGY蛋白转运；本项目实施有利于真菌效应因子转运机制的揭示，并可促进真菌效应因子转运阻断技术的开发。

Plant pathogens manipulate host cell processes for successful colonization through effectors, indicating that the blocking technique based on effectors translocation mechanism has huge potential to plant disease control. However, the studies of fungal effectors translocation lag behind, so far, phytopathologists haven’t found conserved host-targeting signal (HTS) in fungi, and don’t know how fungal effectors translocate across the host plasma membrane..Recently, the study focusing on HTS of oomycete RxLR effectors revealed the mechanism of phosphatidyinositol-3-phosphate mediated translocation. In previous studies, we defined IGY protein family in poplar tree fungal pathogen Marssonina brunnea, which has extensive similarities with RxLR effector family. Therefore, we plan to study the mechanism of IGY proteins translocating across host plasma membrane. Our research proposal include that identifying IGY proteins inside of poplar cell with proteomics technique, verifying the HTS of IGY proteins with protein hybrid method, observing dynamic state of IGY proteins during infection with live-cell imaging technology, analyzing entry approach of IGY proteins into poplar cells with lipidomics and biomolecule interaction methods, blocking IGY proteins translocation with inhibitor and transgenic method. These studies could accelerate the study of fungal effectors translocation mechanism and provide theoretical basis for developing translocation blocking technique of fungal effectors.