松材线虫通过形成抗逆态幼虫（DL3）适应不良环境，以扩散期幼虫（DL4）随松墨天牛迁飞形成新的侵染源。DL3是形成DL4的唯一虫态，阻遏DL3形成可降低DL4虫口，减少初侵染源，因此DL3形成调控路径中的基因可作为潜在的防治靶标。热激转录因子（hsf-1）直接调控DL3形成的多个基因路径。本项目在鉴定到松材线虫Bx-HSF1参与调控DL3形成的基础上拟研究：Bx-HSF1的转录调控及转录激活能力，所识别的顺式作用元件，下游调控基因及信号通路。应用ChIP-Seq技术筛选Bx-HSF1的靶基因，EMSA明确其识别顺式作用元件，酵母双杂交验证Bx-HSF1的转录激活活性，Q-PCR验证靶基因表达特性，原位杂交进行靶基因组织定位，RNAi验证Bx-HSF1及其靶基因的功能以及BX-HSF1在DL3形成路径中的调控作用，系统揭示Bx-HSF1在DL3形成中的转录调控机理，为松材线虫防治提供新视角。

Bursaphelenchus xylophilus, the causative agent of pine wilt disease, survived under the stress by the means of forming dauer larva (DL3), and spread by forming dispersal larva (DL4). The DL4 is molted from DL3. So, the population of DL4 will be reduced, if the DL3 population was reduced. The genes which invovled in the DL3 formation pathway is a potential targets for nematode control. The hsf-1 regulate 4 main signal pathways related to the DL3 forming. Based on our studies about the Bx-HSF1, which is relate to the DL3 forming, the molecular mechanism of Bx-HSF1 regulated signal pathway in B. xylophilus dauer larva forming will be studied. This study will be focused on the transcriptional regulation and activation, the recognition of target sequences, and the down-stream target genes. The methods of EMSA, ChIP-Seq, two-hybrid screening, Q-PCR, RNAi, and insitu-hybridization will be used to uncover the detail function of Bx-HSF1 and its targets. These results may be contributed in revealing the molecular mechanism of Bx-HSF1 regulated signal pathway in B. xylophilus dauer larva forming and provide new perspectives for the pine wood nematode control.