糖尿病严重威胁人类健康，用于连续血糖监测的植介入式电化学葡萄糖传感器是该领域的研究热点，传感器在体内的稳定性是制约连续血糖监测发展的因素。本项目从电化学血糖酶生物传感器的反应原理入手，首先分析研究传感器植入体内后作为重要反应物参与氧化还原反应的氧气的浓度随炎症反应和排异反应的变化规律，探索植入式血糖传感器在体内稳定性下降以及功能失效的机理；在此基础上，首次提出植入式血糖传感器葡萄糖氧化酶复合生物相容性富氧微球的固定化新方法，通过在体微球的氧气缓释，构建植入式传感器内环境供氧的稳定性，解决由于氧气浓度不足和波动引起的传感器信号不稳定；最后，通过对酶固定化材料的羧基化表面修饰，使之与葡萄糖氧化酶之间形成稳定的共价连接，从而更可靠地固定酶，减小由于酶流失引起的传感器的稳定性下降。本项目的研究为实现血糖传感器的长时间稳定植入奠定基础。

Diabetes mellitus is a worldwide public health problem that threatens human’s health. The implantable electrochemical blood glucose sensor used for continuous blood glucose monitoring (CGMS) has been a hot topic in diabetes research. However, the in vivo stability of glucose sensor barriers the development of CGMS. This study investigates the oxygen concentration changes during each period of the inflammatory response and foreign body response after implantation and studies the mechanism of stability decreasing and function failure of the sensor in vivo from the detection principle of electrochemical biosensors. Glucose oxidase and biocompatible oxygen-rich microsphere complex is used to immobilize enzyme and provide a steady oxygen supply around the implanted glucose sensor. This method could solve the signal drift problem caused by oxygen deficit and oxygen fluctuation. Enzyme immobilization nanomaterial is modified to form a stable covalent connection between glucose oxidase. This covalent connection could reduce the stability decreasing caused by enzyme loss. The research of this project lays a foundation for the realization of long time stability of implantable glucose sensor.