南极磷虾资源蕴藏量巨大，营养丰富，是各国竞相研发的食品新资源热点。但磷虾在贮存加工过程中，易受体内酶作用发生“自溶”，甲壳中的氟迁移至肌肉中，致使肌肉中氟含量过高，限制了磷虾蛋白资源的开发利用。磷虾体内酶活性很强，即使在-20℃冻藏条件下，甲壳中的氟仍可继续迁移至肉中，氟在肌肉中与蛋白、碳水化合物等的结合作用方式及内源酶作用下氟的迁移机制亟待阐明。本项目拟研究南极磷虾中内源酶系的组成，筛选影响磷虾中氟迁移的关键酶，研究酶解前后甲壳、肌肉中氟的赋存形态变化，筛查酶作用下氟迁移的影响因素，揭示酶作用于甲壳的催化机制，探求氟迁移的离子通路，构建酶作用下氟迁移的动力学模型。预期研究结果将为南极磷虾的食用安全性评价提供新思路，为贮存加工中氟的控制技术提供理论依据。

Antarctic krill are widely distributed in Central Antarctic waters, as an important part of the food chain, huge reserves of resources, has become the hot spots of the world competing to develop the human food resources. Antarctic krill in the fluorine content is much higher than the food safety limit, During the storage process, in the role of endogenous proteases, krill body produced autolysis. Fluoride in the crustaceans migrate irreversibly to the muscle, resulting in the high fluoride content in the muscle tissue, limiting the development and utilization of krill resources. The activity of enzymes in krill is very strong, even at the temperature of -20℃ storage conditions, fluorine can still migrate to the soft tissue. The action mode of fluorine combined with protein, carbohydrates in soft tissue and fluorine migration mechanism in the autolysis process from shell to soft tissue need to clarify. The project aims to study the composition of endogenous enzymes of Antarctic krill and to screen the key enzyme affecting the fluorine migration of krill, studying the morphological changes of the composition of fluorine after enzymatic hydrolysis, screening the impact factor of the fluorine migration, revealing the enzyme action in crustacean, exploratiing for fluoride ion channel migration, building various types of protease dissolution krill muscle fluorine migration dynamics model. Results are expected to provide a working basis for the follow-up study of Antarctic krill's food safety assessment and processing of fluorine in control technology.