我们前期研究工作表明，猪星状病毒在国内猪群有着较高的流行率，并将阳性材料在PK-15细胞中连续传代分离出两株具有较高生长滴度的猪星状病毒 (PAstV1-NNJG7和PAstV1-GX1)，致病性实验表明病毒感染仔猪出现轻微腹泻，增重减少，说明猪星状病毒危害着猪养殖业。目前，国内外对星状病毒的流行病学和诊断技术的研究较多，尚未对猪星状病毒细胞受体的进行研究的报道。本课题中，拟通过梯度密度离心和多聚物双水相方法提取PK-15细胞质膜，提取膜蛋白。采用病毒铺覆蛋白印迹（VOPBA）、免疫共沉淀（CO-IP）和蛋白化学交联等方法筛选病毒候选受体。再用病毒受体重建、RNAi技术进一步鉴定候选受体。本研究为阐明猪星状病毒定植、分布、传播和致病的分子机制提供科学依据。

Our previous studies showed that high prevalence of porcine astrovirus in swine herds was detected by RT-PCR. Two strains of porcine astrovirus named PAstV1-NNJG7 was isolated and identified in PK-15 cells inoculated with suspension of astrovirus-positive tissue. Piglets challenged with porcine astrovirus (PAstV1-NNJG7 and PAstV1-GX1) showed diarrhea and loss of weigh, threatening development of swine industry. In this proposal, membrane proteins of PK-15 will be isolated and purified by density gradient centrifugation procedures and aqueons two-phase system. The candidate cellular receptor will be screened by VOPBA、CO-IP and emulsion-chemical cross linking method and further identified by RNAi and receptor reconstruction. Our studies will enhance our understanding the location and distribution and transmission and pathogenicity of porcine astrovirus.