产气荚膜梭菌能引起动物坏死性肠炎、人畜气性坏疽和食物中毒。生物被膜不仅使细菌产生严重耐药，还能引起严重的食品安全问题。产气荚膜梭菌生物被膜形成过程是多基因、多蛋白与多信号分子共同作用的复杂过程。目前国内外关于产气荚膜梭菌生物被膜形成的研究工作仅局限于单一基因或蛋白，未见从全基因和全菌蛋白角度对该菌生物被膜分子机制进行研究的报道，这就不能系统地揭示该菌生物被膜形成的分子机制。因此本项目将在前期基础之上采用CLSM、SEM等方法明确青海地区产气荚膜梭菌生物被膜不同生长时期的形态和结构及其与耐药相关性；采用全菌基因差异和全菌蛋白差异比较法对产气荚膜梭菌生物被膜型与浮游型菌株进行研究，获得生物被膜相关基因与蛋白，生物信息学与质谱法分析生物被膜相关基因与蛋白的种类、结构与功能，全面揭示该菌生物被膜分子机制，这将为进一步研究该菌生物被膜基因与信号分子调控机理奠定基础，还可为新型抗菌药物的研发提供依据。

Clostridium perfringens can cause animal necrotic enteritis,human and livestock gas gangrene and food poisoning.Bacterial biofilm not only enable the bacteria to produce serious drug resistance,but also cause serious food safety.Biofilm formation of Clostridium perfringens is a complex interaction process with many genes, proteins and signal molecular.At present,researches on biofilm formation of Clostridium perfringens is limited for single gene or protein in domestic and abroad,there has not been reported on biofilm molecular mechanism of Clostridium perfringens from whole genes and whole bacterial proteins,which can not reveal systematically the biofilm molecular mechanism of Clostridium perfringens.Therefore,on the basis of previous studies,this project will use CLSM, SEM methods to determine biofilm morphology and structure of different growth stages of Clostridium perfringens in Qinghai and its correlation with the drug resistance.Using the gene difference comparison and the whole bacterial protein difference comparison research biofilm and floating type strain of Clostridium perfringens,obtain relevant gene and protein of biofilm formatoin,bioinformatics and mass spectrometry analyze genes' and proteines' species,structure and function of biofilm formation,reveal fully the molecular mechanism of bacterial biofilm,this will lay the foundation for studying further biofilm gene and molecular mechanisms of signal regulation for biofilm of Clostridium perfringens,can also provide a scientific basis for research and development of new antibacterial drug.