我们通过睡眠呼吸暂停模式间隙低氧大鼠氧化应激损机制及抗氧化作用的研究发现，模拟OSAHS患者间隙低氧/再氧和状态的各组浓度间隙低氧大鼠动物模型，其单核细胞、白细以及多核细胞均高于正常组，由此推测广泛存在于单核细胞表面的TLR4可能参与了OSAHS的发生发展。miRNA对TLR4的调控作用部分已经得到证实，但关于其对TLR4调控在OSAHS发生发展中的作用尚不明确。本项目拟研究：1、不同浓度间隙低氧大鼠外周血TLR4的表达水平，阐明TLR4在不同浓度间隙低氧大鼠外周血中的表达水平是否存在差异；2、检测TLR4在不同浓度间歇低氧大鼠肺组织中的定位及表达水平，并与外周血中TLR4表达水平进行比较；3、对不同浓度间隙低氧大鼠血清中miRNA进行表达谱分析，筛选出TLR表达相关miRNA；4、证实miRNA通过调控TLR信号通路参与了OSAHS形成。

We hypoxic rats by sleep apnea model clearance oxidative stress damage mechanism, and the study found that antioxidant effect simulation OSAHS patients with hypoxic/clearance and the concentration of oxygen and status of each clearance hypoxic rat animal model, the monocytes, white fine and multinucleated cells were higher than in normal group, which speculated that widely exists in mononuclear cells on the surface of TLR4 may participate in the development of OSAHS.Micrornas in the readjustment of TLR4 part has been confirmed, but about the TLR4 regulation in its role in the development of OSAHS is unclear.This project intends to research: 1, the different concentration interval hypoxic rat peripheral blood TLR4 expression level of TLR4 in different concentration interval hypoxic rats in the peripheral blood expression level whether there is a difference,2, detection of TLR4 in different concentration of intermittent hypoxia in rat lung tissue localization and expression level, and comparing with TLR4 expression level in peripheral blood;3, with different concentration interval hypoxic rats serum micrornas expression profile analysis, screening TLR expression related micrornas;4, confirm the miRNA through regulating TLR signaling pathways involved in the formation of OSAHS.