臭氧已超过PM2.5成为我国很多地区5~10月份首要环境污染因子。臭氧与光照协同产生的光氧化作用对植物伤害极大。本项目在前期进行了不同葡萄品种臭氧敏感性研究的基础上，利用臭氧发生室进行臭氧与光胁迫协同处理，探讨不同光强下发生臭氧伤害的臭氧浓度阈值；摸清臭氧与强光胁迫发生的先后顺序对气孔运动的影响；引入D1蛋白合成抑制剂氯霉素，测定PSⅡ活性、D1蛋白含量，摸清臭氧与强光协同处理对PSⅡ的破坏机制；引入敌草隆（DCMU）阻断PSII向PSI的电子传递，探讨臭氧胁迫对PSⅠ活性及PSⅠ活性氧清除系统的影响；分析光合碳同化相关酶/基因表达和光合同化产物积累，探讨协同胁迫对光合作用暗反应的影响。测定编码D1蛋白基因psbA 表达，探讨臭氧胁迫在抑制D1蛋白修复中的作用；分析光破坏防御途径光呼吸、叶黄素循环的响应，进而全面揭示臭氧与光协同作用对葡萄光合性能伤害机制，为调整架势缓解臭氧伤害提供理论依据

Ozone, a primary enviromental contamination factor, has become much higher than PM2.5. Synergetic induction of photooxidation by ozone and light hurts grapevine leaves seriously. On the base of suscepbility of different grapevine varieies to zone stress, we treated grapevines with light and ozone stress to clarify the injury threshold valule of ozone under different light intensity, Studied the sequencing effect of light intensity and ozone stress treatment to stomatal movement; PSⅡactivity and D1 protein content were tested to clarify the synergetic effect of light intensity and ozone stress on PSⅡafter treated with chloramphenicol; Studied the ozone stress to PSⅠactivity and the antioxidant system of PSⅠafter DCMU applied; Analyzed the relative enzyme in photosynthetic carbon assimilating to clarify the effect of light intensity and ozone stress on dark reactions; The expression of psbA gene, encoding D1 protein, were analyzed to study the light intensity and ozone stress on the inhibition of D1 repair; Meanwhile, photorespiration and xanthophyll cycle responses were analyzed to clarify the effect of light intensity and ozone stress on photoprotection pathways.Which completely reveal the synergistic effects of light and ozone stress on photosynthetic function in grapevine leaves, and provided theory basis of training system to alleviate ozone injury.