地理分布的差异导致植物形成了不同的生态型，通过研究不同生态型基因组、环境因子、表现型的互作是高通量发掘与适应性相关基因的重要手段。本项目拟采用我国特征鲜明的7个生态型的桃种质资源为材料，首先重点开展气候因子与基因组SNP的相关性分析，获得与各气候因子适应性相关的基因组区段；其次，对基因组受选择区段分析确定各生态型适应自身生存环境的基因组区段；同时，选择适应性性状需冷量和开花期，通过全基因组关联分析获得其关联位点，分析关联位点在不同生态型间的分化，阐明需冷量和开花期与环境的互作位点，以及生态型间变异机制；最后，利用大连（北方）和昆明（南方）种植的相同品种的需冷量和开花期全基因组关联分析，获得稳定关联位点和可变关联位点，稳定位点在自然群体和遗传群体中验证。本研究基于基因组、气候因子、物候期三者的互作发掘与适应性相关的基因，为全基因组水平揭示桃生态适应性的遗传基础提供理论依据。

Plant species are divided into different ecotypes results from the variation of geographic distributions. Approach based on the interactions between genome, climate factors, and phenotypes is the major mathod for adaptation associated genes high-throughput mining. Seven distinct ecotypes of peach collections will be used as materials in this project. Firstly, we will detect the loci associated with each climate factors through the correlation analysis between climate factors and SNPs. Secondly，Through genomic regions undergone selection detecting, we can obtain the genomic regions underlying the adaptive genetic basis of each ecotype. Meanwhile，Given the adaptive traits, flowering time and chilling requirement, the association loci will be detected through a genome-wide association study based on the phenotypes and SNPs. Subsequently, the association loci which are associated with climate adaptation will be detected by searching for loci with elevated differentiation among seven ecotypes. Based on these analysis, we can obtained the loci involved in the interactions between blooming date, chilling requirement and climate. Finally，We will test the robust association loci and alternative association loci by the genome-wide association studies of flowering time and chilling requirement which will be conducted at Dalian (North) and Kunming (South), respectively. Based on that, we will verify the stable SNPs using natural population and genetic population. Our study will conduct high-throughput adaptation associated genes mining through the interaction between genome, climate factors, and phenological phase in peach which will provide theoretical evidences for peach genetic adaptation research at genome-wide level.