CD4+T细胞亚群失衡是乙肝慢性化的主要原因，与DC功能异常有关。大样本研究表明脾虚湿热兼肾虚是慢乙肝基本病机，临床RCT研究证实补肾健脾方是治疗慢乙肝的有效方剂，其疗效与改善DC功能、平衡CD4+T细胞亚群分化有关。前期证实，HBV调节DC模式识别受体（PRRs)表达，抑制CD4+T细胞向Th1分化，促进Th2/Treg分化。基于DC的PRRs诱导CD4+T细胞亚群分化的机制，提出假说:补肾健脾方通过“DC的PRRs-细胞因子-CD4+T细胞分化关键转录因子"途径，平衡免疫细胞网络治疗慢乙肝。应用RCT研究样本库（血清和外周血单个核细胞），筛选与补肾健脾方疗效有关的DC表达的PRRs、细胞因子及CD4+T细胞分化转录因子；应用DC /CD4+T细胞共培养体系建立“HBV→DC的PRRs→细胞因子→关键转录因子→细胞亚群”免疫模型，明确中药作用靶点，阐释补肾健脾方治疗慢乙肝的免疫机制。

The imbalance of CD4+T cell subsets is a major cause of chronicity of hepatitis B, which is associated with the abnormalities of DCs function. Large sample study showed that spleendeficiency and dampness heat and kidney deficiency is the basic pathogenesis of chronic hepatitis B, and the clinical RCT research confirm that Bushen Jianpi Recipe is an effective prescription for the treatment of chronic hepatitis B, its efficacy is associated with the improvement of the function of DC and the balance of CD4 + T cell subsets differentiation. Our previous studies showed that HBV regulated the expression of PRRs in DCs and inhibited the differentiation of CD4+T cells to Th1, and promoted the differentiation of of CD4+T cells to Th2/Treg. Based on the mechanism of the differentiation of CD4+T cell subsets induced by PRRs expressed on DC, the hypothesis was presented: "the recipe treat chronic hepatitis B by balancing immune cell network through the" PRRs on DCs - cytokine - key transcription factor during the differentiation of CD4+T cell "pathway. Sample library of clinical RCT study (serum and peripheral blood mononuclear cells) will be used in this study, PRRs on DCs，cytokines and key transcription factor during the differentiation of CD4+T cell were screened. The coculture system of DC and naive CD4+T cells will be used to build the immune model”HBV- PRRs on DCs - cytokine - key transcription factor during the differentiation of CD4+T cell”, which will help to clarify the target point of traditional Chinese medicine and to explain the immune mechanism of the treatment of chronic hepatitis B with the Bushen Jianpi Recipe