不同于大多数葡萄品种只在果皮呈现色泽，少数葡萄品种果皮和果肉以及其他组织器官都能呈现色泽，被称为调色品种，对葡萄酒调色具有重要作用。项目以欧亚种调色品种‘烟73’（V. vinifera）及其母本非调色品种‘玫瑰香’（V. vinifera）为试材，首先进行不同发育时期各组织器官，即果皮、果肉、果穗轴（含果柄）、叶肉、叶脉及枝条韧皮部的转录组分析，筛选不同组织器官着色和花色苷组分的可能候选调控基因。进而研究重要的外界因子光照条件对果皮、果肉和果穗轴（含果柄）花色苷组分的影响，分析不同花色苷组分对光照的响应以及可能的候选调控基因。最后以其他调色品种和非调色品种为试材，对得到的可能候选调控基因进行实时定量PCR检测，进一步确认调控不同组织器官着色和花色苷组分以及对光照响应的主要基因，并进行初步功能验证。这不仅有助于揭示花色苷组分的基因调控，而且对于培育调色葡萄品种具有重要的应用价值。

Anthocyanins of grape berries are synthesized and accu¬mulated in berry skin of most grape cultivars, however, some grape germplasm have anthocyanins in both skin and pulp, called teinturier cultivars. A teinturier grape ‘Yan 73’ (V. vinifera) and its maternal parent non-teinturier grape ‘Muscat Hamburg’ will be used in this project. First, various organs, including skin, pulp, berry pedicels (including carpopodia), leaf lamina, leaf vein and living bark at the base of the shoot (phloem) during development will be investigated through RNA-sequencing, in order to screen candidate genes that may be responsible for coloration and anthocyanin composition in different organs. Sencond, RNA-seuencing will also applied to investigate differentially expressed candidate genes after sunlight exclusion from skin, pulp and berry pedicels. Last, candidate genes will be further validated in a biologically independent experiment with other teinturier and non-teinturier grapes using quantitative real-time PCR (qRTPCR), and their functions will be tested. This project would provide a deeper understanding of the genetic basis underlying anthocyanin composition in various organs, and the response to sunlight, and would be helpful for more efficient breeding strategies for higher levels of specific anthocyanins.