果树果实发育和成熟调控是果树学研究的核心问题之一， 近年来，草莓逐渐成为非呼吸跃变型果实发育和成熟研究的模式材料。我们以往的研究发现，蔗糖非酵型蛋白激酶FaSnRK2.6在草莓果实发育和成熟调控中起着重要作用，本项目拟在此为线索，以二倍体草莓为试材，首先通过果实瞬时表达体系，系统探讨FaSnRK2s家族中除FaSnRK2.6外，其它所有成员在草莓果实发育和成熟中的作用及机理；然后通过草莓果实特异启动子驱动的稳定遗传体系，进一步确认FaSnRK2s家族中操纵果实发育和成熟调控的核心信号成员，并解析这些信号蛋白之间在草莓果实发育和成熟调控中的协同或冗余调控机制；进而通过多种分子技术，揭示FaSnRK2.6的互作蛋白，解析FaSnRK2.6的上、下游信号蛋白及信号路径，探讨FaSnRK2.6和其它核心成员之间可能存在的协同作用或功能冗余的分子基础。 该研究具有重要的理论价值和一定的实践指导意义。

The study of fruit development and ripening is the one of the most important aspects of fruit ripening research. Recently, more and more studies use the strawberry fruit as the research model of non-climatic fruit ripening research. Our previous studies find that SNF-related serine/threonine-protein kinase, FaSnRK2.6, plays the key role in the regulation of strawberry fruit development and ripening. Based on this result, we will use the diploid strawberry as the material to further discuss the function and the regulation signal mechanism of FaSnRK2s. Firstly, we will use the fruit transient gene expressed assay to analysis the function of all FaSnRKs members except FaSnRK2.6; then we will further confirm the key members and their synergetic or redundant mechanism in the regulation of strawberry fruit development and ripening with the stable transgenic assay; Finally, we will reveal the interaction proteins of FaSnRK2.6, and further explore the upstream, downstream signal proteins and the signal pathways associated with the regulation of FaSnRK2.6 triggered fruit ripening.