申请人自2007年作为“高层次人才”引进到华南农业大学工作以来，一直从事作物根系适应酸性低磷土壤的生理和分子机制研究。对大豆核心种质磷效率与酸性磷酸酶的关系进行了系统评价，初步阐明了主要豆科作物根系磷高效的生理和分子基础。在作物根系磷酸酯酶参与有机磷活化的生理生化基础、苹果酸参与豆科作物根系适应磷铝胁迫的机制、根瘤响应低磷胁迫蛋白的鉴定和功能分析等方面取得了重要研究成果。近五年来在Current Opinion in Biotechnology和Plant Physiology等国内外著名专业杂志上发表论文23篇，其中SCI收录论文17篇，影响因子大于5的SCI论文6篇。以第一或通讯作者发表的8篇代表性论文平均SCI影响因子4.9。主持和主要参加了国家自然科学基金和国家重点基础研究发展计划等项目。本课题拟研究大豆根瘤响应低磷胁迫的特异生理和分子机制，以期阐明豆科作物“以磷促氮”的机理。

I have focused on studying physiological and molecular mechanisms underlying legume root adaptation to phosphorus (P) deficiency on acid soils since I returned from the United States and joined in South China Agricultural University in 1997. We have evaluated the correlation between P efficiency and acid phosphatase (APases) among the applied core collection for phosphorus efficiency of soybean germplasm. We have primarily elucidated the physiological and molecular mechanisms of high P efficiency in roots of main legume crops, especially on characterization of phosphatases involved in organic P mobilization, functions of malate in legume root adaptation to P deficiency and aluminum toxicity, isolation and functional analysis of P deficient responsive proteins in soybean nodules. In the past five years, I have published 23 papers in the famous journals, such as Current Opinion in Biotechnology and Plant Physiology. Among them, 17 papers are cited by SCI. The average impact factor of eight representative papers is 4.9, in which I am the first author or correspondence author. I have presided over or taken part in 8 grants, such as national natural science foundation of China and national key basic research special funds of China. We are going to study specific mechanisms in soybean nodules responsive to P deficiency at both physiological and molecular levels, and thus further understand the mechanisms underlying improved P nutrition caused by increased nitrogen efficiency in legume crops.

根际过程调控是农田水肥高效利用的重要途径，根-土界面的“交流与互作”一直是植物营养学的研究热点。在国家基金委优秀青年科学基金-植物营养学项目的支持下，利用农业化学、植物生理生化、植物分子生物学等方法与手段，针对根系应答根际环境的分子生理机制及其过程调控的作用途径开展一系列的研究工作，取得重要的创新性成果如下：1）剖析了根系14-3-3蛋白及其作用途径应答根际水磷变化的角色，发现14-3-3蛋白GRF9及其调控的质子泵成员AHA2与AHA7在此过程中起重要作用，此结果发表了两篇学术文章且标注了基金资助（Journal of Experimental Botany, 2015, 66 : 6371-6384；Journal of Experimental Botany, 2017, 68 : 1731-1741）；2）建立了自然条件下根系向水性的研究方法，剖析了根系向水性的作用途径，指出油菜素内脂BR受体BRI1蛋白与质子泵AHA2蛋白协同调控的根系质子外排无论在垂直或侧面方向的向水性过程中均起重要作用，此结果获授权发明专利一项，理论方面的认知也被国际知名期刊Plant Physiology接受且标注了基金资助；3）发现干湿交替灌溉可促进水稻根系高效利用水分与磷素，G蛋白与其激素作用因子在此过程中扮演重要角色，此结果发表了三篇学术文章且标注了基金资助（Journal of Experimental Botany, 2015, 66 : 6371-6384；Plant Cell Rep., 2017, 36: 557-569; 中国生物化学与分子生物学报，2017，33：1258-1265），并获得了运盛青年科技奖，有良好地推广与应用价值。综上所述，我们紧紧围绕“根际营养与分子调控”开展工作，正式发表标注基金资助的学术文章5篇，已接受标注基金资助的学术文章1篇，发明专利已授权1项，获得科技奖1项，获批中英人才计划1项，解析了根系应答根际水磷环境变化的工作途径及其分子生理机制，能为通过植物充分利用根际土壤养分与水分提供调控手段，以缓解水分亏缺对农业生产的影响和减轻肥料施用过多带来的环境压力，因此具有重要意义。