脑水肿是中风领域研究的热点和难题。课题组前期以羚角钩藤汤加减治疗中风急性期脑水肿疗效显著，但作用机制仍不清楚。本研究根据中医“治未病”的诊疗理念，结合肢体缺血预适应保护远隔重要脏器的最新认识，提出假说：羚角钩藤汤加减联合肢体缺血预适应，通过综合调整血脑屏障功能治疗中风急性期脑水肿。拟优选肢体缺血预适应相关参数，采用TTC染色、MRI技术、电镜检查等手段评价脑组织病理学变化，并通过免疫组化、Westernblot、qRT-PCR等技术检测血脑屏障Occludin、Claudin-5、MMP-2、MMP-9和AQP-4等关键靶点表达，在组织和分子水平阐明羚角钩藤汤加减联合肢体缺血预适应治疗缺血性脑中风急性期脑水肿的作用机制。通过本项目研究，探索羚角钩藤汤加减治疗中风急性期脑水肿的核心机制，评价肢体缺血预适应作为中医药辅助疗法的可行性，为临床合理用药提供依据，对于指导中医药治疗中风具有重要意义。

Brain edema is a hot and difficult problem in research of stroke. Modified Ling Jiao Gou Teng formula developed by our team for the treatment of stroke, has demonstrated positive therapeutic effect clinically. But the mechanism remains unclear. According to the theory of preventive treatment of traditional Chinese medicine and new findings of the limb ischemia preconditioning protection of vital organs, we hypothesized that Modified Ling Jiao Gou Teng formula combined application of limb ischemia preconditioning is likely to be a cure for stroke throughout its regulatory effect of blood brain barrier (BBB) function. We optimize the best parameters of limb ischemia preconditioning. Brain tissue pathological changes were evaluated by TTC staining, MRI technology, electron microscope examination. Aided with immunohistochemical, Westernblot, qRT-PCR techniques, expression of Occludin, Claudin-5, MMP-2, MMP-9 and AQP-4 in blood brain barrier were detected to investigate the mechanism of reducing brain edema in the early brain injury, which were treated by Modified Ling Jiao Gou Teng formula combined application of limb ischemia preconditioning. Through this project research, we explore the core mechanism of treatment stroke in acute phase by Modified Ling Jiao Gou Teng formula, and evaluate the feasibility of limb ischemia preconditioning as adjuvant therapy of traditional Chinese medicine. Besides, this project can provide evidence for reasonable clinical administration of drugs and guidance for Chinese medicine treatment of stroke.