烟粉虱是世界性重大农业害虫，探讨破坏素对烟粉虱免疫反应的抑制机理，为基于昆虫免疫系统信号分子为靶标设计新农药提供新的思路。在前期研究破坏素对斜纹夜蛾SL-1细胞的致凋亡作用和对小菜蛾免疫相关差异表达基因Serpins的作用，以及测定分析烟粉虱转录组和蛋白质谱数据的基础上，本项目利用生物信息学结合基因芯片技术筛选烟粉虱的免疫相关基因/蛋白；测定破坏素处理烟粉虱的表达谱和蛋白质谱，筛选免疫破坏素和被破坏素抑制的免疫相关关键因子；利用RNAi技术阐明关键因子在细胞免疫或体液免疫中的相关功能；利用荧光原位杂交(FISH)，Northern blots结合Western blots揭示被破坏素抑制的关键免疫因子的功能，从而阐明破坏素抑制烟粉虱免疫反应的机制。研究结果可为以害虫免疫系统为靶标的免疫抑制剂的开发奠定理论基础，最终为我国农业的可持续发展提供害虫控制的新策略和新方法。

Sweetpotao whitifly, Bemisia tabaci is a majorglobal spread pest. To explore the mechanism of destrucxin's suppression on the immune response of Bemisia tabaci can provide the discovery of novel biological insecticide with new idea. Based on destruxins possessing a preferable activity for apoptosis at the cellular level of Spodoptera litura SL-1 cells, significant effects on the expression of serine protease inhibitor genes (serpins) in the larvae of Plutella xylostella, and the transcriptome and protein mass spectrometry, this program combines bioinformatics with genechip technology to filter outimmune related genes/proteins. The gene expression pattern and protein profiles will be performed to compare andanalyze the key factorswhich respond to or are oppressed by destruxin. The function of critical factors in cellular immunity and humoral immunity will be demonstrated bythe RNA interference technology. The fluorescence in situ hybridization (FISH), Northern blots and Western blot will help us to reveal the functions of these significant immune factors, and then to clarify the potential molecular mechanism of the immune response to destruxinin Bemisia tabaci. This study will benefit the development of immune inhibitors that target pest immune system. A greener way to produce food and a more sustainable development of controlling pests will be available eventually.