红苞凤梨嵌合叶由绿色和白色组织形成金边、金心等多种类型，且花果艳丽多彩，观赏期长，是重要的新型观赏植物。但其嵌合性状在繁殖过程中易发生变异甚至消失，大量再生植株因丧失观赏价值而被淘汰。前期测序发现5个叶绿素合成相关基因在白化细胞中下调表达，其中hemC是导致白化的关键基因。植物表观遗传学己成为当今植物科学研究新的焦点，非编码RNA调控是表观遗传调控的重要内容。本项目将在前期研究的基础上，以组培分离的全绿、全白植株为材料，利用高通量测序构建两种细胞类型的miRNA和lncRNA表达谱并进行表达差异分析；筛选调控细胞白化关键基因表达的关键调控非编码RNA；检测关键调控因子及其靶基因的表达模式和靶基因的DNA甲基化水平；采用CRISPR-Cas9基因敲除、超表达验证关键调控因子功能。探明关键调控因子的结构、功能及相互作用网络，为红苞凤梨金边嵌合性状的的分子改良和稳定性调控奠定基础。

Variegated chimera varieties of Ananas bracteatus have been planted as important landscape plants for its ornamental values of color leaf and fruit. The leaves are composed of green normal tissues and abino tissues. However the variegated chimera character of Bromeliad is unstable. Many plants regenerated by in vitro tissue culture lost ornamental value for the losing of leaf color. It is very important and meaningful to study the molecular mechanism of chimera. Our studies showed that the compressing of the gene expression and synthesis of chlorophyll lead to the chlorosis of the cells. The hemC is the key gene playing an important role the cell chlorosis. Study on epigenetic regulation mechanism is becoming the new research hot point in plant science, and the non-coding RNA regulation is the most important content of epigenetic regulation. Based on our preliminary studies, the complete green or white plants derived from in vitro culture were used in the further study of epigenetic regulation mechanism of the key genes leading to the chlorosis of cells in the applied project. The miRNA and lncRNA expression profiles were constructed by Illumina sequence and the variations between the green plant and white plant were analyzed. The key non-coding RNAs working in cell chlorosis were screened by correlation analysis. The expression patterns of the key non-coding RNAs and their target genes were detected by Real-time PCR and western blot. The DNA-methylation of the target genes was analyzed. The biological function of the key non-coding RNAs were verified by CRISPR-Cas9 gene knockout and gene over expression technique. This study could illuminate the structure, function and interaction network of the key non-coding RNA in cell chlorosis. The research results could lay a foundation of the molecular improvement and stability regulation of the chimera character of Ananas bracteatus.