转录因子在植物生长发育和胁迫应答反应中起着重要的调控作用。课题组研究发现从番茄中克隆的一个MADS-box转录因子ShMADSTF同时参与番茄对活体寄生真菌（白粉菌）和腐生真菌（黄萎菌）的抗性反应。但目前MADS-box转录因子参与植物抗病性报道较少。基于MADS-box转录因子新的重要生物学功能的推测，本项目拟进一步对番茄转录因子ShMADSTF进行研究：采用转基因的方法鉴定该转录因子在植物抗病反应中的功能；VIGS方法分析其参与激素信号途径；借助酵母双杂交系统鉴定与转录因子ShMADSTF直接互作的蛋白分子，分析其在植物抗性反应中参与的PTI和ETI信号通路；采用ChIP-Seq方法获得转录因子ShMADSTF在番茄全基因组的结合谱，生物信息学分析其调控分子机理。最终阐释番茄转录因子ShMADSTF的功能及抗病分子机制，为进一步选育和改良植物抗病新品种奠定理论基础。

Transcription factors can regulate many genes in plant growth and development and adverse stresses. In previous study, a MADS-box transcription factor named as ShMADSTF was cloned from tomato, which was participated in tomato resistance response to biotrophic fungi powdery mildew and nectrophic fungi Verticillium wilt. But there is few report that MADS-box transcription factor participated in plant disease resistance at present. Based on the hypothesis of MADS-box transcription factor involving in new important biological function, further function analysis on ShGSTU1 will be conducted including: the over-expression ShMADSTF tomato is gained by transgenic method, and diease-resistance function of which are identified; plant hormone signaling pathways of whihch were studied by VIGS; the tomato powdery mildew effectors and endogenous signal molecules interaction with ShMADSTF are identified by applying yeast two-hybrid method to analyze the PTI (PAMP triggered innate immunity) and ETI (effector triggered immunity) signaling pathways which involved in plant immune response; the whole genomic combining spectrum of ShMADSTF was gained by ChIP-Seq method, the molecular regulation mechanism of which was analyzed by bioinformatics. All the results will well elucidate the function and resistance mechanism of tomato transcription factor ShMADSTF and provide theoretical basis for plant breeding and improvement of new resistance varieties.