本项目针对生物样品中违禁药物的富集和检测中存在的操作繁琐、选择性差、灵敏度低，且难以实现对多种结构类似物同时富集检测等问题，提出通过对目前社会危害严重的吗啡类违禁药物开展示范性研究，探索出一条以磁性碳纳米管表面分子印迹技术为导向的复杂基质中违禁药物富集与检测的新思路。首先基于计算机模拟和逆向预测技术制备出具有高亲和力、高选择性、快速结合动力学的两亲性磁性碳纳米管表面分子印迹材料；然后将其作为磁性固相萃取中的特异性吸附剂，充分发挥磁性材料在磁场下易于操控、碳纳米管比表面积和机械强度高、分子印迹材料选择性高的优势，通过与全自动磁珠提取纯化系统及超高效液相色谱-高分辨质谱联用，构建针对吗啡类违禁药物及其结构类似物、代谢产物的高通量、自动化、高灵敏度分析平台，实现对富集材料和分析方法的技术创新，并有望用于指导兽用违禁药物分析、兴奋剂检测、法庭毒物分析等研究，促进功能材料的发展和分离分析技术的进步。

Conventional treatments for biosamples need tedious procedures, the selectivity and sensitivity toward illegal drugs is often poor, and simultaneously determination could hardly been made. To face these problems, representative study on the morphine detection was proposed to explore the thought of illegal drugs enrichment and detection in complex matrice which guided by surface imprinting technology based on magnetic carbon nanotubes (MCNTs). First, amphiphilic imprinting material on MCNTs was prepared according to the outcome of computer simulation and backward forecasting. The imprinting material should perform highly affinity and selectivity, fast binding kinetics. Then, it could be exploited as a magnetic absorbent, while we taking its preponderances of manipulability under magnet, high specific area and mechanical strength of CNTs, high selectivity of molecular imprinting material. Last, an automatic, high throughput and sensitive assay would be built which are employing fully automatic magnetic purification system and ultra performance liquid chromatography tandem mass-spectrometry. This assay is aimed at determining morphine, its structural derivatives and metabolite. It would actualize technical innovation of analyte enriching material and analytical methods, and we are looking forward its usage in veterinary illegal drugs analysis, doping control technology, forensic toxicological analysis, etc., and eventually promotion of functional materials and separation analysis technologies.

本项目按照预定研究计划，针对生物样品中违禁药物的富集和检测中存在的操作繁琐、选择性差、灵敏度低，且难以实现对多种结构类似物同时富集检测等问题，提出通过对目前社会危害严重的吗啡类违禁药物开展示范性研究，探索出一条以磁性碳纳米管表面分子印迹技术为导向的复杂基质中违禁药物富集与检测的新思路。首先基于计算机模拟和逆向预测技术制备出具有高亲和力、高选择性、快速结合动力学的两亲性磁性碳纳米管表面分子印迹材料；然后将其作为磁性固相萃取中的特异性吸附剂，充分发挥磁性材料在磁场下易于操控、碳纳米管比表面积和机械强度高、分子印迹材料选择性高的优势，通过与全自动磁珠提取纯化系统及超高效液相色谱联用，构建针对吗啡类违禁药物及其结构类似物、代谢产物的高通量、自动化、高灵敏度分析平台，实现对富集材料和分析方法的技术创新，促进功能材料的发展和分离分析技术的进步。迄今为止在国外高水平学术刊物上发表学术论文8篇，协助培养硕士研究生3名。