周围神经损伤后骨骼肌失神经萎缩的防治是世界性难题。研究表明，骨髓间充质干细胞（BMSCs）能分化成神经元样结构、分泌多种神经营养因子及抗炎细胞因子，有一定延缓失神经骨骼肌萎缩的作用，但不能防止肌肉粘连和关节僵硬。中医推拿手法可以促进失神经骨骼肌卫星细胞分裂增殖，增强乙酰胆碱酶活性，减缓运动终板退变，延缓失神经骨骼肌萎缩，并能够有效防止失神经肌肉粘连和关节僵硬，已广泛应用于临床，但单一方法防治失神经骨骼肌萎缩的作用均有限。本研究通过推拿手法与BMSCs移植联合应用，利用行为学、形态学和电生理等方法，研究推拿手法联合BMSCs移植对防治失神经骨骼肌萎缩及促进肢体功能恢复的影响，并通过酶组织化学和分子生物学方法研究Pax7基因与肌卫星细胞之间的关系，探讨推拿手法与BMSCs移植联合应用在防治失神经骨骼肌萎缩中的机制，为推拿手法联合BMSCs移植防治失神经骨骼肌萎缩的临床应用提供理论依据。

The prevention and treatment of skeletal muscle denervation atrophy after peripheral nerve injury is a worldwide problem. Previous studies indicated that bone marrow mesenchymal stem cells (BMSCs) can differentiate into neuron like structures and secrete various neurotrophic factors and anti-inflammatory cytokines, which play an important role in delaying the atrophy of denervated skeletal muscle. Traditional Chinese massage can promote the skeletal muscle satellite cell proliferation, increase the activity of acetylcholine, and slow down the motor endplate degeneration, delaying denervated skeletal muscle atrophy, and has been widely applied in clinical practice. However, relying on a single method is producing only limited effects on stopping denervated skeletal muscle atrophy. In this study, traditional Chinese massage is practiced in combination with BMSCs transplantation, while behavioral, morphological and electrophysiological methods are employed to study the effects of massage combined with BMSCs transplantation on denervated skeletal muscle atrophy. Enzyme histochemical and molecular biological methods are also used to study the relationship between Pax7 gene and muscle satellite cells, with the purpose of exploring the mechanism and providing a theoretical basis for the clinical application of massage combined with BMSCs transplantation for the prevention and treatment of skeletal muscle atrophy.