藏医对类风湿性关节炎( RA )有深入而系统的认识。根据藏医“干黄水”治疗理论和临床疗效，二十五味驴血丸是藏医治疗RA的主要药物，但是目前尚未见到有关其作用机制方面的研究报道。根据藏医理论和前期实验研究结果，我们推测：二十五味驴血丸通过抑制Th17细胞过度增殖、提高Treg细胞免疫抑制作用，使Th17/Treg达到平衡，从而达到治疗RA的作用。本项目拟以目前公认的CIA大鼠作为类风湿关节炎模型，用藏医经典方剂--二十五味驴血丸进行干预，从调控Th17/Treg细胞平衡的角度，探讨二十五味驴血丸治疗RA的机制。本研究不仅可以部分揭示二十五味驴血丸治疗RA的机理，也可为藏医“干黄水”治疗RA提供实验依据和科学内涵；因此，具有重要的理论意义和较高的实用价值。

Tibetan medicine had a deep and systematic understanding on rheumatoid arthritis. Ershiwuwei Lvxue Pill is the major drug which is used to cure rheumatoid arthritis according to the tibetan medicine theory of “ drying up yellow liquid ” and clinical efficacy. However, there is no research report about its mechanism now. Based on the tibetan medicine theory and the earlier experimental data, we hypothesized that rheumatoid arthritis could be cured by the equilibrium of Th17/Treg cells which was reached by Ershiwuwei Lvxue Pill through inhibiting the excessive proliferation of Th17 cells and enhancing immunosuppression function of Treg cells. The aim of this study is to explore the therapeutic effect of Ershiwuwei Lvxue Pill to rat collagen-induced arthritis (CIA) and its possible molecular mechanism from the balance of Th17/Treg cells with acknowledged CIA rats as the RA model. This study will not only help reseachers to understand the mechanism of Ershiwuwei Lvxue Pill effecting on rheumatoid arthritis, but also provide experimental basis and scientifical connotation for the “ drying up yellow liquid ” theory. In summary, this study had important theoretical and practical value.