感染性休克是 ICU 患者主要致死病因, 病死率高达 15% ~50%，国际上3次更新严重脓毒症和感染性休克指南。2013年新版指南强调复苏必须达到目标，并给予量化目标——输注30 mL/kg液体。现今存在的问题是：患者存在个体差异性，30 mL/kg液体对部分感染性休克患者复苏液量是过度的。为寻找个体化精确指导感染性休克容量复苏的生物标记物，本研究拟从感染性休克过度液体复苏导致静脉回流障碍问题切入，制作感染性休克过度容量复苏动物模型和对照组相比，首先应用Guyton定律量化分析静脉回流系统参数改变、其次用微透析方法评估组织代谢改变、最后分析循环microRNA表达谱，寻找感染性休克过度容量复苏时特异性循环microRNA表达。做该microRNA表达水平和静脉回流系统参数相关分析，期望找到特异性microRNA做为个体精细化指导感染性休克容量复苏的的生物学标记物，提高感染性休克治愈率。

Septic shock is a major cause of ICU patients, mortality is as high as 15% ~ 50%, three times update guide for severe sepsis and septic shock on the international. The new guideline in 2013 worldwide cause a strong resonance, emphasized the resuscitation must reach the goal, and gave advice quantitative objectives - 30 mL/kg crystal fluid infusion. Today the main problems existing in the clinical, guideline to give quantitative objectives facilitate global extension, but we find in the clinical work of 30 mL/kg liquid crystal for some patients with septic shock resuscitation fluid volume is excessive, the goal can't satisfy for the individualized treatment. Looking for effective, precise guide individualized septic shock fluid resuscitation of the biomarkers, this study start from the septic shock excessive liquid recovery cause venous return obstacle problems. We make animal model of septic shock excess fluid resuscitation with control group; the first we use the Guyton’s law quantitative analysis deterioration of venous return system parameter changes; the second microdialysis method will be used to assess organizational metabolic changes; in the final, analysis circulating microRNA expression spectrum. Then do the microRNA expression level with the Guyton's law of venous return system parameter correlation analysis. Expect to find the specific microRNA as individual refinement guidance the biological markers of fluid resuscitation with septic shock patient, for improving septic shock recovery rate.