高分子量谷蛋白亚基(HMW-GS)是影响小麦加工品质的重要因素。通过EMS诱导分别敲除优质弱筋小麦宁麦9号的5个HMW-GS，利用回交转育法获得了单亚基缺失的近等基因系。利用此近等基因系，在不同施氮量和施氮模式下，通过定量PCR和谷蛋白分步分离量化技术，研究灌浆期籽粒HMW-GS编码基因表达、HMW-GS表达量和谷蛋白聚合体质量与数量的动态变化，分析其对小麦籽粒面团流变学特性和加工品质的效应。解析不同氮肥管理下HMW-GS缺失对弱筋小麦品质形成的影响及机制，为利用HMW-GS缺失提高弱筋小麦品质提供理论依据和方法。

End-use quality of wheat is mainly determined by high-molecular-weight glutenin subunit (HMW-GS). A set of near-isogenic lines (NILs) with single HMW-GS deletion was developed by EMS-induced and backcross method on Ningmai 9 background, which was characterized with typical weak-gluten quality. Based on the NILs, field trials will be conducted with nitrogen application rate and splitting pattern. The dynamic change of HMW-GS gene expression, the quantity of HMW-GS, and the quality and quantity of glutenin polymeric protein will be investigated. Dough rheological properties and end-use quality of harvested grain will be also evaluated. This project will clarify the mechanism of the effect of HMW-GS deletion on weak-gluten wheat quality under different nitrogen application regimes. The results will provide theoretical basis and methodology for making use of HMW-GS deletion in weak gluten wheat production.