植物中具有大量不同的矮化基因及其矮化机制。在水稻、拟兰芥和小麦等植物中的矮化基因主要导致植物体内的激素代谢失衡，阻碍细胞分化和伸长，从而导致矮化。也有少部分的矮化基因引起植物木质素或类黄酮的代谢失衡导致矮化。小麦中发现和定位了超过20个矮化基因，很少矮化基因的矮化机制被了解，且主要引起赤霉素代谢失衡导致矮化。DPW的矮化涉及茎秆伸长期木质素、类黄酮、SAM、SAH和细胞纤维素等合成途径的某些蛋白，不同于赤霉素合成途径，但该矮化基因及其生理和分子机制未知。本研究利用精细定位、目的区域捕获测序、图位克隆、代谢组学等方法相结合，分析分离DPW的Rht-dp矮化基因，利用转基因功能验证、蛋白质杂交、基因定量表达、酵母双杂交、组织化学染色等方法了解其分子机制以及生理机制。

Different mechanisms of plant dwarf or semi-dwarf genes, primarily involved in plant phytohormone biosynthesis, transport and signaling, have been/being studied and discovered. In addition to these phytohormone-related dwarfing genes and mechanisms, several dwarfing genes, unbalanced lignin or flavonoid metabolisms, also cause dwarfisms in rice, tobacco, and Brachypodium distachyon. In wheat, more than 20 dwarfing genes were discovered and mapped. A few of dwarfing genes, involved in GA biosynthesis, transport or signaling, were studied. Other dwarfing genes and their mechanisms are unknown. Transcriptomic and proteomic analysis revealed that Dwarf polish wheat dwarfing genes, Rht-dp mapped on 4BS, were involved in lignin, flavonoid, SAM, SAH and cellulose metabolisms, which was different from dwarfing genes caused the unbalance of GA metabolism. However, the sequence of Rht-dp, and its molecular function and physiological mechanism are still unknown. Based on our previous studies, we will perform fine mapping and targeted next generation sequencing to clone Rht-dp, and use transgenic technology, metabolomics, western blot, qRT-PCR, yeast double hybridization and histochemical straining to reveal its molecular and physiological mechanisms.