稻瘟病是水稻生产上最重要的病害之一，发掘抗稻瘟病基因并应用于抗病品种选育是经济有效的防控途径，而我国在粳稻资源抗稻瘟病基因发掘和利用方面仍较为薄弱。申请人前期选用辽宁病害重发区多年多点表现广谱高抗性的粳稻材料港育129，利用Super-BSA技术定位到一个抗病基因Pi65(t)，位于第11染色体30.2-31.2M间，该基因在不同背景下可表现出稳定的高抗性，但Pi65(t)是否为新的抗病基因类型，其通过参与何种代谢途径调控水稻对稻瘟病菌的抗性等仍不明确。为此，本申请拟在前期基础上深入开展以下研究：1）利用回交群体鉴定重组子，精细定位该基因；2）根据基因功能注释和表达模式确定候选基因；3）通过遗传转化验证候选基因功能，并结合转录组测序技术研究该基因的调控谱。该研究对揭示抗病基因Pi65(t)的抗病机制，发掘和利用抗稻瘟病基因资源，进而选育抗稻瘟病粳稻品种具有重要的科学意义和实用价值。

Abstract：Rice Blast is one of the most important diseases in rice production. To control the disease, detecting rice blast resistance genes and breeding some good varieties is a cost-effective way. Present our research is weak in detecting and utilizing rice blast resistance genes from japonica rice resources. In the previous study, using Gangyu129, a japonica rice cultivar with broad-spectrum resistance in high incidence disease area of Liaoning province, Pi65(t) have been mapped and located to 30.2-31.2M in Chr11, and many resources with the gene under different genetic background have stable and highly resistance to rice blast. Despite the progress in previous research, it is unclear that if Pi65(t) is a new type of gene and the pathway it regulate. Based on previous studies, this research tries to carry out the followed studies in depth: The first, we want to cross the backcross populations and select recombinants to map the gene deeply. The second, we try to forecast the candidate genes according functional annotation and patterns of gene expression. The third, we want to prove the genes' function by genetic transformation and detect the regulatory pathway of the gene using RNA-seq technique. Our study is scientifically and practically important to detect rice blast resistance genes, clarify the resistance mechanism of Pi65(t) and breed japonica rice varieties with resistance to rice blast disease.