由Xanthomonas campestris pv. vesicatoria (Xcv) 引起的番茄细菌性疮痂病国内外发生比较严重，缺乏安全有效的防治措施。利用harpin蛋白PopW可有效减少Xcv的定殖。Harpin蛋白作为生物激发子可诱导植物广谱抗病性，其诱导植物抗病机制的研究过去主要集中在水杨酸, 茉莉酸/乙烯等信号通路的解析方面，但对于harpin蛋白如何与植物互作影响病原细菌侵入的机制鲜有报道。前期研究发现PopW可诱导番茄早期免疫相关marker基因的转录增强，抑制Xcv诱导番茄叶片气孔张开。因此，本项目将通过研究气孔在PopW诱导番茄早期免疫反应抵抗Xcv侵入中的功能，分析PopW诱导气孔免疫信号途径，最终解析PopW诱导番茄抵抗Xcv侵入的机制。本项目研究结果将为番茄细菌性疮痂病的控制提供理论和技术支撑。

Bacterial spot disease caused by Xanthomonas campestris pv. vesicatoria in tomato is very serious due to the lack of effective control measures. PopW, as a harpin protein, can repress X. campestris pv. vesicatoria. Harpin can function as elicitor to induce the plant resistance against pathogens, through salicylic acid, jasmonic acid/ethylene signal pathways and their cross-talk. But how harpin interacts with plant to restrict the early infection of bacterial pathogen is not clear. In the previous study, it was found that PopW can induce enhanced transcription of tomato innate immunity associated genes and repress stomatal opening induced by Xcv. To elucidate the mechanism of PopW-induced plant innate immunity to restrict the early infection of Xcv in tomato, the role of stomata and the signal pathway will be studied in PopW-triggered plant immunity against Xcv invasion. This study will provide theoretical and technical support for the control of X. campestris pv. vesicatoria.