Priming是激发子诱导采后果实抗病性的一种普遍形式，其赋予果实在病原菌侵染时展现更强更快抗病反应的能力，但其分子调控机理还不清楚。本项目拟在前期研究的基础上，以采后桃果实为试材，以化学激发子（MeJA、BTH和BABA）诱导的桃果实Priming反应中泛素连接酶E3与转录因子PpWRKY2对抗病性的调控作用及其互作机制为核心研究内容，从挖掘桃果实功能性含RING结构泛素连接酶E3入手，分析功能性泛素连接酶E3和转录因子PpWRKY2的生物活性，研究泛素连接酶E3和转录因子PpWRKY2在桃果实Priming抗病网络中的调控作用，探索PpWRKY2转录因子作为泛素连接酶E3靶蛋白的可能性并解析两者之间的互作模式，以期从蛋白调控的角度阐明桃果实Priming抗病信号网络，揭示Priming抗病反应的分子调控机理，为基于Priming现象的诱导抗病技术在果实采后保鲜中的应用提供科学依据。 

Priming response has been recognized as a common feature of the defense response induced by various elicitors in harvested fruits, where the fruits can express a faster and stronger activation of specific disease resistance after they have been infected by a pathogen. However, the molecular basis of defense priming is poorly understood. In current study, with the typical climacteric fruit peaches as the test material, the regulatory effect and interactive mechanism of ubiquitin ligase E3 and transcription factors (TF) PpWRKY2 associated with the priming response induced by elicitors such as BTH, MeJA and BABA in harvested peaches will be investigated and served as the core research content. Beginning with the exploit of ubiquitin ligase E3 containing RING structure, the present study is going to analyze the biological activity of the functional ubiquitin ligase E3 and TF PpWRKY2 and to explore the regulatory effects on the network of priming defense in peaches. Afterwards, the possibility of TF PpWRKY2 as the target protein of the ubiquitin ligase E3 and interaction between them will be determined, which will finally lead to the construction of the regulatory network of the defense priming based on protein regulation and elucidate the molecular regulation mechanism of priming response. This study will lay the foundation for the commercial use of induced disease resistance in postharvest storage of fruits based on priming mechanism.