夜行性昆虫趋光行为过程中光源敏感性和上灯节律是如何形成的，一直是昆虫学领域研究热点之一，但仍是尚未解决的科学难题。研究表明昆虫视蛋白具有光源敏感性，且对行为节律有调控作用。那么视蛋白是如何调控夜行性昆虫光源敏感性和上灯节律的呢？这是我们感兴趣的科学问题。项目组前期从棉铃虫成虫头部转录组分析中获得了7种视蛋白基因序列。本项目拟克隆棉铃虫7种视蛋白基因，检测其时空表达特征，通过RNAi沉默视蛋白基因后检测棉铃虫光源敏感性和上灯节律的变化，并明确光源选择及节律相关基因trp、stops、nianaC、NinaA、per、tim、cry等的表达与视蛋白基因表达的关系，从而阐明视蛋白对棉铃虫趋光行为的调控作用和机制，对丰富和完善昆虫趋光性理论，推动灯光诱杀技术中光源的改进和基于上灯节律确定开关灯时间，从而提高灯光诱杀效果和选择性，促进害虫物理防治产业的发展具有重要的理论和实践意义。

Studies on the mechanism of light source preference and phototaxis rhythm among nocturnal insect has been one of the hotspots in entomology, but it still has many aspects that remain unknown. A large number of studies have shown that opsin determine insects’ selectivity to wavelength, which has a regulatory function on insect rhythm behavior. We focused on how opsin determined selectivity wavelength and phototaxis rhythm behavior of insects. We acquired seven opsin genes from the adult head transcriptome of Helicoverpa armigera analysis. In the study we clone seven opsin genes of H. armigera and then measure their mRNA levels in spatio-temporal using quantitative real-time PCR. We also study the light selection and phototaxis rhythm of H. armigera after silencing opsin genes using RNAi. Meanwhile, we define the correlation between opsin genes’ spatio-temporal expressions and related genes (trp、stops、nianaC、NinaA、per、tim、cry ) of light selection and rhythm expression pattern. The results would help us understand the opsin regulation mechanisms and effects in H. armigera phototaxis behavior, enrich and perfect light selection theory; benefit light trapping efficiency and selectivity by improving light source of light trapping techniques and regulating lights switch based on the phototaxis rhythm; and promote the development of physical control pests industry.